This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2001-01-19    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
NOT hydroxymethylpyrimidine kinase activity	IGI: Inferred from Genetic Interaction with SGD:THI21, SGD:THI22 Assigned on 2009-09-07	Kawasaki Y, et al.  (2005) Biosynthesis of hydroxymethylpyrimidine pyrophosphate in Saccharomyces cerevisiae. Curr Genet 47(3):156-62	SGD
phosphomethylpyrimidine kinase activity	ISA: Inferred from Sequence Alignment with EBI:P76422 Assigned on 2009-09-07	Llorente B, et al.  (1999) Genetic redundancy and gene fusion in the genome of the Baker's yeast Saccharomyces cerevisiae: functional characterization of a three-member gene family involved in the thiamine biosynthetic pathway. Mol Microbiol 32(6):1140-52	SGD
NOT phosphomethylpyrimidine kinase activity	IGI: Inferred from Genetic Interaction with SGD:THI21, SGD:THI22 Assigned on 2009-09-07	Kawasaki Y, et al.  (2005) Biosynthesis of hydroxymethylpyrimidine pyrophosphate in Saccharomyces cerevisiae. Curr Genet 47(3):156-62	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
thiamine biosynthetic process	IEP: Inferred from Expression Pattern Assigned on 2001-01-18	Llorente B, et al.  (1999) Genetic redundancy and gene fusion in the genome of the Baker's yeast Saccharomyces cerevisiae: functional characterization of a three-member gene family involved in the thiamine biosynthetic pathway. Mol Microbiol 32(6):1140-52	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular_component unknown	ND: No Biological Data Available Assigned on 2001-01-19	SGD  (2002) Use of the ND evidence code for Gene Ontology (GO) terms in SGD ()	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for THI22

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
thiamine biosynthetic process	IEA: Inferred from Electronic Annotation with EBI:IPR004399 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
extracellular region	IEA: Inferred from Electronic Annotation with EBI:SL-0243 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0964 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).