This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2006-08-09    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
cyclin-dependent protein serine/threonine kinase regulator activity	IDA: Inferred from Direct Assay Assigned on 2013-03-29	Schwob E, et al.  (1994) The B-type cyclin kinase inhibitor p40SIC1 controls the G1 to S transition in S. cerevisiae. Cell 79(2):233-44	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
G1/S transition of mitotic cell cycle	IEP: Inferred from Expression Pattern Assigned on 2001-01-18 IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Schwob E and Nasmyth K  (1993) CLB5 and CLB6, a new pair of B cyclins involved in DNA replication in Saccharomyces cerevisiae. Genes Dev 7(7A):1160-75	SGD
G2/M transition of mitotic cell cycle	IEP: Inferred from Expression Pattern Assigned on 2002-07-10 IMP: Inferred from Mutant Phenotype Assigned on 2002-07-10	Schwob E and Nasmyth K  (1993) CLB5 and CLB6, a new pair of B cyclins involved in DNA replication in Saccharomyces cerevisiae. Genes Dev 7(7A):1160-75	SGD
positive regulation of DNA replication	IMP: Inferred from Mutant Phenotype Assigned on 2006-08-09	Schwob E and Nasmyth K  (1993) CLB5 and CLB6, a new pair of B cyclins involved in DNA replication in Saccharomyces cerevisiae. Genes Dev 7(7A):1160-75	SGD
positive regulation of spindle pole body separation	IGI: Inferred from Genetic Interaction with SGD:CLB4, SGD:CLB3, SGD:ASE1 Assigned on 2009-02-18	Crasta K, et al.  (2006) Cdk1 regulates centrosome separation by restraining proteolysis of microtubule-associated proteins. EMBO J 25(11):2551-63	SGD
	IGI: Inferred from Genetic Interaction with SGD:CLB4, SGD:CLB3 Assigned on 2009-02-13	Yeong FM, et al.  (2001) Early expressed Clb proteins allow accumulation of mitotic cyclin by inactivating proteolytic machinery during S phase. Mol Cell Biol 21(15):5071-81	SGD
premeiotic DNA replication	IGI: Inferred from Genetic Interaction Assigned on 2002-07-10 IMP: Inferred from Mutant Phenotype Assigned on 2002-07-10	Stuart D and Wittenberg C  (1998) CLB5 and CLB6 are required for premeiotic DNA replication and activation of the meiotic S/M checkpoint. Genes Dev 12(17):2698-710	SGD
regulation of cyclin-dependent protein serine/threonine kinase activity	IDA: Inferred from Direct Assay Assigned on 2013-03-29	Schwob E, et al.  (1994) The B-type cyclin kinase inhibitor p40SIC1 controls the G1 to S transition in S. cerevisiae. Cell 79(2):233-44	SGD
spindle assembly	IGI: Inferred from Genetic Interaction Assigned on 2006-08-09 IMP: Inferred from Mutant Phenotype Assigned on 2006-08-09	Schwob E and Nasmyth K  (1993) CLB5 and CLB6, a new pair of B cyclins involved in DNA replication in Saccharomyces cerevisiae. Genes Dev 7(7A):1160-75	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IDA: Inferred from Direct Assay Assigned on 2002-07-10	Jacobson MD, et al.  (2000) Testing cyclin specificity in the exit from mitosis. Mol Cell Biol 20(13):4483-93	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for CLB5

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein kinase binding	IEA: Inferred from Electronic Annotation with EBI:IPR014400 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cell division	IEA: Inferred from Electronic Annotation with EBI:KW-0132 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
regulation of cell cycle	IEA: Inferred from Electronic Annotation with EBI:IPR014400 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
regulation of cyclin-dependent protein serine/threonine kinase activity	IEA: Inferred from Electronic Annotation with EBI:IPR014400 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:IPR004367 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).