This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2002-07-05    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
cyclin-dependent protein serine/threonine kinase regulator activity	IMP: Inferred from Mutant Phenotype Assigned on 2013-03-29	Surana U, et al.  (1991) The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae. Cell 65(1):145-61	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2013-03-29 IDA: Inferred from Direct Assay Assigned on 2013-03-29	Amon A, et al.  (1992) Regulation of p34CDC28 tyrosine phosphorylation is not required for entry into mitosis in S. cerevisiae. Nature 355(6358):368-71	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
G2/M transition of mitotic cell cycle	IEP: Inferred from Expression Pattern Assigned on 2001-01-18 IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Grandin N and Reed SI  (1993) Differential function and expression of Saccharomyces cerevisiae B-type cyclins in mitosis and meiosis. Mol Cell Biol 13(4):2113-25	SGD
positive regulation of spindle pole body separation	IGI: Inferred from Genetic Interaction with SGD:CLB4, SGD:CLB3, SGD:CLB1 Assigned on 2009-02-13	Fitch I, et al.  (1992) Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae. Mol Biol Cell 3(7):805-18	SGD
regulation of cyclin-dependent protein serine/threonine kinase activity	IMP: Inferred from Mutant Phenotype Assigned on 2013-03-29	Surana U, et al.  (1991) The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae. Cell 65(1):145-61	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular bud neck	IDA: Inferred from Direct Assay Assigned on 2005-06-02	Bailly E, et al.  (2003) Differential cellular localization among mitotic cyclins from Saccharomyces cerevisiae: a new role for the axial budding protein Bud3 in targeting Clb2 to the mother-bud neck. J Cell Sci 116(Pt 20):4119-30	SGD
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2005-06-02	Bailly E, et al.  (2003) Differential cellular localization among mitotic cyclins from Saccharomyces cerevisiae: a new role for the axial budding protein Bud3 in targeting Clb2 to the mother-bud neck. J Cell Sci 116(Pt 20):4119-30	SGD
	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-07-05 IMP: Inferred from Mutant Phenotype Assigned on 2002-07-05	Hood JK, et al.  (2001) The Saccharomyces cerevisiae cyclin Clb2p is targeted to multiple subcellular locations by cis- and trans-acting determinants. J Cell Sci 114(Pt 3):589-97	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2005-06-02	Bailly E, et al.  (2003) Differential cellular localization among mitotic cyclins from Saccharomyces cerevisiae: a new role for the axial budding protein Bud3 in targeting Clb2 to the mother-bud neck. J Cell Sci 116(Pt 20):4119-30	SGD
	IDA: Inferred from Direct Assay Assigned on 2002-07-05	Hood JK, et al.  (2001) The Saccharomyces cerevisiae cyclin Clb2p is targeted to multiple subcellular locations by cis- and trans-acting determinants. J Cell Sci 114(Pt 3):589-97	SGD
spindle	IDA: Inferred from Direct Assay Assigned on 2005-06-02	Bailly E, et al.  (2003) Differential cellular localization among mitotic cyclins from Saccharomyces cerevisiae: a new role for the axial budding protein Bud3 in targeting Clb2 to the mother-bud neck. J Cell Sci 116(Pt 20):4119-30	SGD
spindle pole body	IDA: Inferred from Direct Assay Assigned on 2005-06-02	Bailly E, et al.  (2003) Differential cellular localization among mitotic cyclins from Saccharomyces cerevisiae: a new role for the axial budding protein Bud3 in targeting Clb2 to the mother-bud neck. J Cell Sci 116(Pt 20):4119-30	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for CLB2

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein kinase binding	IEA: Inferred from Electronic Annotation with EBI:IPR014400 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cell division	IEA: Inferred from Electronic Annotation with EBI:KW-0132 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitosis	IEA: Inferred from Electronic Annotation with EBI:KW-0498 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
regulation of cell cycle	IEA: Inferred from Electronic Annotation with EBI:IPR014400 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
regulation of cyclin-dependent protein serine/threonine kinase activity	IEA: Inferred from Electronic Annotation with EBI:IPR014400 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:IPR004367 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).