This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2004-08-13    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ARF guanyl-nucleotide exchange factor activity	IDA: Inferred from Direct Assay Assigned on 2002-10-16	Jones S, et al.  (1999) Genetic interactions in yeast between Ypt GTPases and Arf guanine nucleotide exchangers. Genetics 152(4):1543-56	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
exocytosis	IGI: Inferred from Genetic Interaction with SGD:YPT32, SGD:YPT31 Assigned on 2008-08-01	Jones S, et al.  (1999) Genetic interactions in yeast between Ypt GTPases and Arf guanine nucleotide exchangers. Genetics 152(4):1543-56	SGD
positive regulation of ARF protein signal transduction	IMP: Inferred from Mutant Phenotype Assigned on 2011-01-06 IDA: Inferred from Direct Assay Assigned on 2011-01-06	Chen KY, et al.  (2010) Syt1p promotes activation of Arl1p at the late Golgi to recruit Imh1p. J Cell Sci 123(Pt 20):3478-89	SGD
vesicle-mediated transport	IGI: Inferred from Genetic Interaction Assigned on 2005-04-26	Jones S, et al.  (1999) Genetic interactions in yeast between Ypt GTPases and Arf guanine nucleotide exchangers. Genetics 152(4):1543-56	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
Golgi apparatus part	IDA: Inferred from Direct Assay Assigned on 2011-01-06	Chen KY, et al.  (2010) Syt1p promotes activation of Arl1p at the late Golgi to recruit Imh1p. J Cell Sci 123(Pt 20):3478-89	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-08-13	Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ARF guanyl-nucleotide exchange factor activity	IEA: Inferred from Electronic Annotation with EBI:IPR000904 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
guanyl-nucleotide exchange factor activity	IEA: Inferred from Electronic Annotation with EBI:KW-0344 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
phospholipid binding	IEA: Inferred from Electronic Annotation with EBI:IPR001849 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
regulation of ARF protein signal transduction	IEA: Inferred from Electronic Annotation with EBI:IPR000904 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).