This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2002-09-30    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ribosomal large subunit binding	IDA: Inferred from Direct Assay Assigned on 2006-07-20	Si K and Maitra U  (1999) The Saccharomyces cerevisiae homologue of mammalian translation initiation factor 6 does not function as a translation initiation factor. Mol Cell Biol 19(2):1416-26	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IMP: Inferred from Mutant Phenotype Assigned on 2007-07-31	Basu U, et al.  (2001) The Saccharomyces cerevisiae TIF6 gene encoding translation initiation factor 6 is required for 60S ribosomal subunit biogenesis. Mol Cell Biol 21(5):1453-62	SGD
maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IMP: Inferred from Mutant Phenotype Assigned on 2007-07-31	Basu U, et al.  (2001) The Saccharomyces cerevisiae TIF6 gene encoding translation initiation factor 6 is required for 60S ribosomal subunit biogenesis. Mol Cell Biol 21(5):1453-62	SGD
ribosomal large subunit biogenesis	IMP: Inferred from Mutant Phenotype Assigned on 2008-07-11	Wood LC, et al.  (1999) Cloning of murine translation initiation factor 6 and functional analysis of the homologous sequence YPR016c in Saccharomyces cerevisiae. J Biol Chem 274(17):11653-9	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2002-09-30	Basu U, et al.  (2001) The Saccharomyces cerevisiae TIF6 gene encoding translation initiation factor 6 is required for 60S ribosomal subunit biogenesis. Mol Cell Biol 21(5):1453-62	SGD
	IPI: Inferred from Physical Interaction Assigned on 2005-04-07	Horsey EW, et al.  (2004) Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. RNA 10(5):813-27	SGD
ribosomal subunit export from nucleus	IGI: Inferred from Genetic Interaction with SGD:SDO1 Assigned on 2007-03-23	Menne TF, et al.  (2007) The Shwachman-Bodian-Diamond syndrome protein mediates translational activation of ribosomes in yeast. Nat Genet 39(4):486-95	SGD
rRNA processing	IGI: Inferred from Genetic Interaction with SGD:SDO1 Assigned on 2007-03-23	Menne TF, et al.  (2007) The Shwachman-Bodian-Diamond syndrome protein mediates translational activation of ribosomes in yeast. Nat Genet 39(4):486-95	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2002-09-30	Basu U, et al.  (2001) The Saccharomyces cerevisiae TIF6 gene encoding translation initiation factor 6 is required for 60S ribosomal subunit biogenesis. Mol Cell Biol 21(5):1453-62	SGD
nucleolus	IDA: Inferred from Direct Assay Assigned on 2007-03-20	Menne TF, et al.  (2007) The Shwachman-Bodian-Diamond syndrome protein mediates translational activation of ribosomes in yeast. Nat Genet 39(4):486-95	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2002-09-30	Basu U, et al.  (2001) The Saccharomyces cerevisiae TIF6 gene encoding translation initiation factor 6 is required for 60S ribosomal subunit biogenesis. Mol Cell Biol 21(5):1453-62	SGD
preribosome, large subunit precursor	IDA: Inferred from Direct Assay Assigned on 2009-01-09	Harnpicharnchai P, et al.  (2001) Composition and functional characterization of yeast 66S ribosome assembly intermediates. Mol Cell 8(3):505-15	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-08-25	Volta V, et al.  (2005) Sen34p depletion blocks tRNA splicing in vivo and delays rRNA processing. Biochem Biophys Res Commun 337(1):89-94	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
preribosome, large subunit precursor	IDA: Inferred from Direct Assay Assigned on 2008-08-25	Fuentes JL, et al.  (2007) In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1. Mol Genet Genomics 278(1):105-23	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ribosomal large subunit binding	IEA: Inferred from Electronic Annotation with HAMAP:MF_03132 Last updated 2013-03-02	Swiss Institute of Bioinformatics (SIB) curators and GOA curators  (2006) Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins	UniProtKB
ribosome binding	IEA: Inferred from Electronic Annotation with EBI:IPR002769 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
translation initiation factor activity	IEA: Inferred from Electronic Annotation with HAMAP:MF_03132 Last updated 2013-03-02	Swiss Institute of Bioinformatics (SIB) curators and GOA curators  (2006) Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0396 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
mature ribosome assembly	IEA: Inferred from Electronic Annotation with EBI:IPR002769 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with HAMAP:MF_03132 Last updated 2013-03-02	Swiss Institute of Bioinformatics (SIB) curators and GOA curators  (2006) Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins	UniProtKB
ribosomal large subunit biogenesis	IEA: Inferred from Electronic Annotation with HAMAP:MF_03132 Last updated 2013-03-02	Swiss Institute of Bioinformatics (SIB) curators and GOA curators  (2006) Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins	UniProtKB
ribosomal subunit export from nucleus	IEA: Inferred from Electronic Annotation with HAMAP:MF_03132 Last updated 2013-03-02	Swiss Institute of Bioinformatics (SIB) curators and GOA curators  (2006) Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins	UniProtKB
ribosome biogenesis	IEA: Inferred from Electronic Annotation with EBI:KW-0690 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
translation	IEA: Inferred from Electronic Annotation with EBI:KW-0648 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
translational initiation	IEA: Inferred from Electronic Annotation with HAMAP:MF_03132 Last updated 2013-03-02	Swiss Institute of Bioinformatics (SIB) curators and GOA curators  (2006) Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0396 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with HAMAP:MF_03132 Last updated 2013-03-02	Swiss Institute of Bioinformatics (SIB) curators and GOA curators  (2006) Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleolus	IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with HAMAP:MF_03132 Last updated 2013-03-02	Swiss Institute of Bioinformatics (SIB) curators and GOA curators  (2006) Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).