This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-05-09    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
phosphatidylinositol phospholipase C activity	IDA: Inferred from Direct Assay Assigned on 2008-05-09	Jun Y, et al.  (2004) Diacylglycerol and its formation by phospholipase C regulate Rab- and SNARE-dependent yeast vacuole fusion. J Biol Chem 279(51):53186-95	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-09	Yoko-o T, et al.  (1995) Isolation and characterization of temperature-sensitive plc1 mutants of the yeast Saccharomyces cerevisiae. Mol Gen Genet 247(2):148-56	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-05-09	Flick JS and Thorner J  (1993) Genetic and biochemical characterization of a phosphatidylinositol-specific phospholipase C in Saccharomyces cerevisiae. Mol Cell Biol 13(9):5861-76	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-09	Coccetti P, et al.  (1998) The PLC1 encoded phospholipase C in the yeast Saccharomyces cerevisiae is essential for glucose-induced phosphatidylinositol turnover and activation of plasma membrane H+-ATPase. Biochim Biophys Acta 1405(1-3):147-54	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
inositol phosphate biosynthetic process	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-09	York JD, et al.  (1999) A phospholipase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA export. Science 285(5424):96-100	SGD
phospholipid catabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-09	Yoko-o T, et al.  (1995) Isolation and characterization of temperature-sensitive plc1 mutants of the yeast Saccharomyces cerevisiae. Mol Gen Genet 247(2):148-56	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-05-09	Flick JS and Thorner J  (1993) Genetic and biochemical characterization of a phosphatidylinositol-specific phospholipase C in Saccharomyces cerevisiae. Mol Cell Biol 13(9):5861-76	SGD
protein localization to kinetochore	IMP: Inferred from Mutant Phenotype Assigned on 2009-02-17	Desai P, et al.  (2009) Plc1p is required for proper chromatin structure and activity of the kinetochore in Saccharomyces cerevisiae by facilitating recruitment of the RSC complex. Mol Genet Genomics 281(5):511-23	SGD
signal transduction involved in filamentous growth	IGI: Inferred from Genetic Interaction with SGD:RAS2 Assigned on 2008-05-09 IPI: Inferred from Physical Interaction with SGD:GPR1, SGD:GPA2 Assigned on 2008-05-09	Ansari K, et al.  (1999) Phospholipase C binds to the receptor-like GPR1 protein and controls pseudohyphal differentiation in Saccharomyces cerevisiae. J Biol Chem 274(42):30052-8	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
chromosome, centromeric region	IDA: Inferred from Direct Assay Assigned on 2008-05-09	DeLillo N, et al.  (2003) Genetic evidence for a role of phospholipase C at the budding yeast kinetochore. Mol Genet Genomics 269(2):261-70	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-05-09	Lin H, et al.  (2000) Phospholipase C is involved in kinetochore function in Saccharomyces cerevisiae. Mol Cell Biol 20(10):3597-607	SGD
condensed nuclear chromosome kinetochore	IPI: Inferred from Physical Interaction with SGD:CEP3, SGD:CBF2 Assigned on 2008-05-09	Lin H, et al.  (2000) Phospholipase C is involved in kinetochore function in Saccharomyces cerevisiae. Mol Cell Biol 20(10):3597-607	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for PLC1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
calcium ion binding	IEA: Inferred from Electronic Annotation with EBI:IPR011992, EBI:IPR002048 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0378 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
phosphatidylinositol phospholipase C activity	IEA: Inferred from Electronic Annotation with EBI:IPR001192, EBI:IPR001711 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:3.1.4.11 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
phosphoric diester hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:IPR017946 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
signal transducer activity	IEA: Inferred from Electronic Annotation with EBI:KW-0807 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
intracellular signal transduction	IEA: Inferred from Electronic Annotation with EBI:IPR001192, EBI:IPR001711 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
lipid catabolic process	IEA: Inferred from Electronic Annotation with EBI:KW-0442 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
lipid metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR017946, EBI:IPR001192, EBI:IPR001711 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0443 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
signal transduction	IEA: Inferred from Electronic Annotation with EBI:IPR001711 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0807 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).