This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2010-05-13    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity	IDA: Inferred from Direct Assay Assigned on 2010-05-13	Stoops JK and Wakil SJ  (1978) The isolation of the two subunits of yeast fatty acid synthetase. Biochem Biophys Res Commun 84(1):225-31	SGD
3-oxoacyl-[acyl-carrier-protein] synthase activity	IMP: Inferred from Mutant Phenotype Assigned on 2010-05-13	Knobling A, et al.  (1975) Malonyl and palmityl transferase-less mutants of the yeast fatty-acid-synthetase complex. Eur J Biochem 56(2):359-67	SGD
contributes_to fatty acid synthase activity	IMP: Inferred from Mutant Phenotype Assigned on 2010-05-13	Qureshi AA, et al.  (1976) Subunits of fatty acid synthetase complexes. Comparative study of enzyme activities and properties of the half-molecular weight nonidentical subunits of fatty acid synthetase complexes obtained from rat, human, and chicken liver and yeast. Arch Biochem Biophys 177(2):364-78	SGD
	IGI: Inferred from Genetic Interaction Assigned on 2010-05-13	Fichtlscherer F, et al.  (2000) A novel function of yeast fatty acid synthase. Subunit alpha is capable of self-pantetheinylation. Eur J Biochem 267(9):2666-71	SGD
holo-[acyl-carrier-protein] synthase activity	IGI: Inferred from Genetic Interaction Assigned on 2010-05-13 IMP: Inferred from Mutant Phenotype Assigned on 2007-01-16	Fichtlscherer F, et al.  (2000) A novel function of yeast fatty acid synthase. Subunit alpha is capable of self-pantetheinylation. Eur J Biochem 267(9):2666-71	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
fatty acid biosynthetic process	IMP: Inferred from Mutant Phenotype Assigned on 2010-05-13	Schuller HJ, et al.  (1992) Differential proteolytic sensitivity of yeast fatty acid synthetase subunits alpha and beta contributing to a balanced ratio of both fatty acid synthetase components. Eur J Biochem 203(3):607-14	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytosol	IDA: Inferred from Direct Assay Assigned on 2007-08-03	Klein HP, et al.  (1967) Fatty acid synthetase of Saccharomyces cerevisiae. J Bacteriol 93(6):1966-71	SGD
fatty acid synthase complex	IDA: Inferred from Direct Assay Assigned on 2007-08-02	Qureshi AA, et al.  (1976) Subunits of fatty acid synthetase complexes. Comparative study of enzyme activities and properties of the half-molecular weight nonidentical subunits of fatty acid synthetase complexes obtained from rat, human, and chicken liver and yeast. Arch Biochem Biophys 177(2):364-78	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2007-08-03	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-09-28	Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity	IEA: Inferred from Electronic Annotation with IUBMB:1.1.1.100 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
3-oxoacyl-[acyl-carrier-protein] synthase activity	IEA: Inferred from Electronic Annotation with IUBMB:2.3.1.41 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
catalytic activity	IEA: Inferred from Electronic Annotation with EBI:IPR016039, EBI:IPR016038 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0511 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
fatty-acyl-CoA synthase activity	IEA: Inferred from Electronic Annotation with IUBMB:2.3.1.86 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
holo-[acyl-carrier-protein] synthase activity	IEA: Inferred from Electronic Annotation with EBI:IPR002582, EBI:IPR008278, EBI:IPR004568 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
magnesium ion binding	IEA: Inferred from Electronic Annotation with EBI:IPR002582, EBI:IPR008278, EBI:IPR004568 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:IPR016040 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
oxidoreductase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0560 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity	IEA: Inferred from Electronic Annotation with EBI:IPR001227 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
fatty acid biosynthetic process	IEA: Inferred from Electronic Annotation with EBI:IPR002582, EBI:IPR004568 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0275 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
fatty acid metabolic process	IEA: Inferred from Electronic Annotation with EBI:KW-0276 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
lipid metabolic process	IEA: Inferred from Electronic Annotation with EBI:KW-0443 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
macromolecule biosynthetic process	IEA: Inferred from Electronic Annotation with EBI:IPR008278 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR016039, EBI:IPR016035, EBI:IPR001227, EBI:IPR016038 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0511 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
oxidation-reduction process	IEA: Inferred from Electronic Annotation with EBI:KW-0560 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).