This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2013-06-18    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
aldehyde dehydrogenase [NAD(P)+] activity	IMP: Inferred from Mutant Phenotype Assigned on 2007-11-27	Meaden PG, et al.  (1997) The ALD6 gene of Saccharomyces cerevisiae encodes a cytosolic, Mg(2+)-activated acetaldehyde dehydrogenase. Yeast 13(14):1319-27	SGD
	IDA: Inferred from Direct Assay Assigned on 2013-06-18	Wang X, et al.  (1998) Molecular cloning, characterization, and potential roles of cytosolic and mitochondrial aldehyde dehydrogenases in ethanol metabolism in Saccharomyces cerevisiae. J Bacteriol 180(4):822-30	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
acetate biosynthetic process	IMP: Inferred from Mutant Phenotype Assigned on 2003-02-13	Eglinton JM, et al.  (2002) Decreasing acetic acid accumulation by a glycerol overproducing strain of Saccharomyces cerevisiae by deleting the ALD6 aldehyde dehydrogenase gene. Yeast 19(4):295-301	SGD
NADPH regeneration	IGI: Inferred from Genetic Interaction with SGD:ZWF1 Assigned on 2009-07-07	Grabowska D and Chelstowska A  (2003) The ALD6 gene product is indispensable for providing NADPH in yeast cells lacking glucose-6-phosphate dehydrogenase activity. J Biol Chem 278(16):13984-8	SGD
response to salt stress	IMP: Inferred from Mutant Phenotype Assigned on 2009-07-08	Butcher RA and Schreiber SL  (2004) Identification of Ald6p as the target of a class of small-molecule suppressors of FK506 and their use in network dissection. Proc Natl Acad Sci U S A 101(21):7868-73	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytosol	IMP: Inferred from Mutant Phenotype Assigned on 2009-08-21	Meaden PG, et al.  (1997) The ALD6 gene of Saccharomyces cerevisiae encodes a cytosolic, Mg(2+)-activated acetaldehyde dehydrogenase. Yeast 13(14):1319-27	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-01-04	Rinnerthaler M, et al.  (2006) MMI1 (YKL056c, TMA19), the yeast orthologue of the translationally controlled tumor protein (TCTP) has apoptotic functions and interacts with both microtubules and mitochondria. Biochim Biophys Acta 1757(5-6):631-8	SGD
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2007-01-04	Rinnerthaler M, et al.  (2006) MMI1 (YKL056c, TMA19), the yeast orthologue of the translationally controlled tumor protein (TCTP) has apoptotic functions and interacts with both microtubules and mitochondria. Biochim Biophys Acta 1757(5-6):631-8	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-08-13	Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
aldehyde dehydrogenase (NAD) activity	IEA: Inferred from Electronic Annotation with IUBMB:1.2.1.3 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
oxidoreductase activity	IEA: Inferred from Electronic Annotation with EBI:IPR016161, EBI:IPR015590, EBI:IPR016162, EBI:IPR016160, EBI:IPR016163 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0560 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor	IEA: Inferred from Electronic Annotation with EBI:IPR016163 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
ethanol catabolic process	IEA: Inferred from Electronic Annotation with UniPathway:UPA00780 Last updated 2013-03-02	UniProt-GOA  (2012) Gene Ontology annotation based on UniPathway vocabulary mapping.	UniPathway
metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR016161, EBI:IPR015590, EBI:IPR016162, EBI:IPR016160, EBI:IPR016163 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
oxidation-reduction process	IEA: Inferred from Electronic Annotation with EBI:IPR016161, EBI:IPR015590, EBI:IPR016162, EBI:IPR016160, EBI:IPR016163 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0560 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).