This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2010-11-12    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
transcription factor binding	IDA: Inferred from Direct Assay Assigned on 2002-10-17	Olson KA, et al.  (2000) Two regulators of Ste12p inhibit pheromone-responsive transcription by separate mechanisms. Mol Cell Biol 20(12):4199-209	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
negative regulation of invasive growth in response to glucose limitation	IGI: Inferred from Genetic Interaction with SGD:DIG2 Assigned on 2010-11-08	Cook JG, et al.  (1996) Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae. Genes Dev 10(22):2831-48	SGD
	IGI: Inferred from Genetic Interaction with SGD:DIG2 Assigned on 2010-11-08	Tedford K, et al.  (1997) Regulation of the mating pheromone and invasive growth responses in yeast by two MAP kinase substrates. Curr Biol 7(4):228-38	SGD
negative regulation of pseudohyphal growth	IGI: Inferred from Genetic Interaction with SGD:DIG2 Assigned on 2010-11-08	Cook JG, et al.  (1996) Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae. Genes Dev 10(22):2831-48	SGD
	IGI: Inferred from Genetic Interaction with SGD:DIG2 Assigned on 2010-11-08	Tedford K, et al.  (1997) Regulation of the mating pheromone and invasive growth responses in yeast by two MAP kinase substrates. Curr Biol 7(4):228-38	SGD
negative regulation of transcription from RNA polymerase II promoter by pheromones	IGI: Inferred from Genetic Interaction with SGD:DIG2 Assigned on 2010-11-08	Bardwell L, et al.  (1998) Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins. Proc Natl Acad Sci U S A 95(26):15400-5	SGD
	IGI: Inferred from Genetic Interaction with SGD:DIG2 Assigned on 2010-11-08	Tedford K, et al.  (1997) Regulation of the mating pheromone and invasive growth responses in yeast by two MAP kinase substrates. Curr Biol 7(4):228-38	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IDA: Inferred from Direct Assay Assigned on 2010-11-03	Cook JG, et al.  (1996) Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae. Genes Dev 10(22):2831-48	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-06-26	Tedford K, et al.  (1997) Regulation of the mating pheromone and invasive growth responses in yeast by two MAP kinase substrates. Curr Biol 7(4):228-38	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Molecular Function

High-throughput Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA binding	IPI: Inferred from Physical Interaction Assigned on 2005-03-31	Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Cellular Component

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).