This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-02-07    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
cyclin-dependent protein serine/threonine kinase activity	IDA: Inferred from Direct Assay Assigned on 2011-02-07	Chi Y, et al.  (2001) Negative regulation of Gcn4 and Msn2 transcription factors by Srb10 cyclin-dependent kinase. Genes Dev 15(9):1078-92	SGD
	ISS: Inferred from Sequence or structural Similarity with SGD:CDC28 Assigned on 2011-01-31	Kuchin S, et al.  (1995) Cyclin-dependent protein kinase and cyclin homologs SSN3 and SSN8 contribute to transcriptional control in yeast. Proc Natl Acad Sci U S A 92(9):4006-10	SGD
RNA polymerase II carboxy-terminal domain kinase activity	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-07 IDA: Inferred from Direct Assay Assigned on 2011-02-07	Hengartner CJ, et al.  (1998) Temporal regulation of RNA polymerase II by Srb10 and Kin28 cyclin-dependent kinases. Mol Cell 2(1):43-53	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
negative regulation of filamentous growth	IGI: Inferred from Genetic Interaction with SGD:STE12 Assigned on 2011-02-07 IMP: Inferred from Mutant Phenotype Assigned on 2011-02-07	Nelson C, et al.  (2003) Srb10/Cdk8 regulates yeast filamentous growth by phosphorylating the transcription factor Ste12. Nature 421(6919):187-90	SGD
negative regulation of transcription from RNA polymerase II promoter	IPI: Inferred from Physical Interaction with SGD:TUP1 Assigned on 2011-02-01	Zaman Z, et al.  (2001) Interaction of a transcriptional repressor with the RNA polymerase II holoenzyme plays a crucial role in repression. Proc Natl Acad Sci U S A 98(5):2550-4	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-01-24	van de Peppel J, et al.  (2005) Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets. Mol Cell 19(4):511-22	SGD
nuclear-transcribed mRNA catabolic process, non-stop decay	IMP: Inferred from Mutant Phenotype Assigned on 2009-03-06	Wilson MA, et al.  (2007) A genomic screen in yeast reveals novel aspects of nonstop mRNA metabolism. Genetics 177(2):773-84	SGD
phosphorylation of RNA polymerase II C-terminal domain	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-07 IDA: Inferred from Direct Assay Assigned on 2011-02-07	Hengartner CJ, et al.  (1998) Temporal regulation of RNA polymerase II by Srb10 and Kin28 cyclin-dependent kinases. Mol Cell 2(1):43-53	SGD
positive regulation of transcription from RNA polymerase II promoter by galactose	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-07	Larschan E and Winston F  (2005) The Saccharomyces cerevisiae Srb8-Srb11 complex functions with the SAGA complex during Gal4-activated transcription. Mol Cell Biol 25(1):114-23	SGD
protein destabilization	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-07	Nelson C, et al.  (2003) Srb10/Cdk8 regulates yeast filamentous growth by phosphorylating the transcription factor Ste12. Nature 421(6919):187-90	SGD
protein phosphorylation	IMP: Inferred from Mutant Phenotype Assigned on 2011-01-24	van de Peppel J, et al.  (2005) Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets. Mol Cell 19(4):511-22	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mediator complex	IDA: Inferred from Direct Assay Assigned on 2011-01-24	Borggrefe T, et al.  (2002) A complex of the Srb8, -9, -10, and -11 transcriptional regulatory proteins from yeast. J Biol Chem 277(46):44202-7	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-02-07	Liu Y, et al.  (2001) Yeast nuclear extract contains two major forms of RNA polymerase II mediator complexes. J Biol Chem 276(10):7169-75	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Molecular Function | Biological Process

High-throughput Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein kinase activity	IDA: Inferred from Direct Assay Assigned on 2012-05-04	Ptacek J, et al.  (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84	SGD

High-throughput Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein phosphorylation	IDA: Inferred from Direct Assay Assigned on 2012-05-04	Ptacek J, et al.  (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR002290 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cyclin-dependent protein serine/threonine kinase activity	IEA: Inferred from Electronic Annotation with IUBMB:2.7.11.22 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
kinase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0418 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein kinase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR002290 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
protein serine/threonine kinase activity	IEA: Inferred from Electronic Annotation with EBI:IPR008271 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0723 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
RNA polymerase II carboxy-terminal domain kinase activity	IEA: Inferred from Electronic Annotation with IUBMB:2.7.11.23 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
transferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity, transferring phosphorus-containing groups	IEA: Inferred from Electronic Annotation with EBI:IPR011009 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
meiosis	IEA: Inferred from Electronic Annotation with EBI:KW-0469 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
phosphorylation	IEA: Inferred from Electronic Annotation with EBI:KW-0418 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein phosphorylation	IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR002290, EBI:IPR008271 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).