This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-06-13    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATPase activity	ISS: Inferred from Sequence or structural Similarity with SGD:RPT6 Assigned on 2011-06-13	Russell SJ, et al.  (1996) Isolation and characterization of SUG2. A novel ATPase family component of the yeast 26 S proteasome. J Biol Chem 271(51):32810-7	SGD
protein domain specific binding	IDA: Inferred from Direct Assay Assigned on 2011-06-13	Chang C, et al.  (2001) The Gal4 activation domain binds Sug2 protein, a proteasome component, in vivo and in vitro. J Biol Chem 276(33):30956-63	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
ER-associated protein catabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2010-09-13	Lipson C, et al.  (2008) A proteasomal ATPase contributes to dislocation of endoplasmic reticulum-associated degradation (ERAD) substrates. J Biol Chem 283(11):7166-75	SGD
nucleotide-excision repair	IGI: Inferred from Genetic Interaction with SGD:RAD23 Assigned on 2011-06-13	Gillette TG, et al.  (2001) The 19S complex of the proteasome regulates nucleotide excision repair in yeast. Genes Dev 15(12):1528-39	SGD
positive regulation of RNA polymerase II transcriptional preinitiation complex assembly	IMP: Inferred from Mutant Phenotype Assigned on 2011-06-13	Malik S, et al.  (2009) The 19 s proteasome subcomplex establishes a specific protein interaction network at the promoter for stimulated transcriptional initiation in vivo. J Biol Chem 284(51):35714-24	SGD
positive regulation of transcription elongation from RNA polymerase II promoter	IMP: Inferred from Mutant Phenotype Assigned on 2010-05-27	Ferdous A, et al.  (2001) The 19S regulatory particle of the proteasome is required for efficient transcription elongation by RNA polymerase II. Mol Cell 7(5):981-91	SGD
proteasome regulatory particle assembly	IMP: Inferred from Mutant Phenotype Assigned on 2010-05-26	Park S, et al.  (2009) Hexameric assembly of the proteasomal ATPases is templated through their C termini. Nature 459(7248):866-70	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IDA: Inferred from Direct Assay Assigned on 2003-02-07	Russell SJ, et al.  (1999) Subcellular localization, stoichiometry, and protein levels of 26 S proteasome subunits in yeast. J Biol Chem 274(31):21943-52	SGD
proteasome regulatory particle, base subcomplex	IDA: Inferred from Direct Assay Assigned on 2002-08-23	Fu H, et al.  (2001) Subunit interaction maps for the regulatory particle of the 26S proteasome and the COP9 signalosome. EMBO J 20(24):7096-107	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-05-26	Glickman MH, et al.  (1998) A subcomplex of the proteasome regulatory particle required for ubiquitin-conjugate degradation and related to the COP9-signalosome and eIF3. Cell 94(5):615-23	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for RPT4

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:IPR003959, EBI:IPR003960 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:IPR005937 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleoside-triphosphatase activity	IEA: Inferred from Electronic Annotation with EBI:IPR003593 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:IPR003593 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein catabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR005937 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:IPR005937 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
proteasome complex	IEA: Inferred from Electronic Annotation with EBI:KW-0647 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).