This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2002-11-13    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
GTPase activity	IMP: Inferred from Mutant Phenotype Assigned on 2002-11-13	Singer-Kruger B, et al.  (1994) Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast. J Cell Biol 125(2):283-98	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-03-24	Will E, et al.  (2001) Expression, purification, and biochemical properties of Ypt/Rab GTPase-activating proteins of Gyp family. Methods Enzymol 329:50-8	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
endocytosis	IMP: Inferred from Mutant Phenotype Assigned on 2002-11-13	Singer-Kruger B, et al.  (1994) Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast. J Cell Biol 125(2):283-98	SGD
Golgi to endosome transport	IGI: Inferred from Genetic Interaction with SGD:YPT52 Assigned on 2013-05-20 IMP: Inferred from Mutant Phenotype Assigned on 2013-05-20	Nickerson DP, et al.  (2012) Termination of isoform-selective Vps21/Rab5 signaling at endolysosomal organelles by Msb3/Gyp3. Traffic 13(10):1411-28	SGD
	IGI: Inferred from Genetic Interaction with SGD:YPT52 Assigned on 2013-05-20 IMP: Inferred from Mutant Phenotype Assigned on 2013-05-20	Paulsel AL, et al.  (2013) Vps9 family protein Muk1 is the second Rab5 guanosine nucleotide exchange factor in budding yeast. J Biol Chem ()	SGD
late endosome to vacuole transport via multivesicular body sorting pathway	IGI: Inferred from Genetic Interaction with SGD:YPT52 Assigned on 2013-05-20	Nickerson DP, et al.  (2012) Termination of isoform-selective Vps21/Rab5 signaling at endolysosomal organelles by Msb3/Gyp3. Traffic 13(10):1411-28	SGD
multivesicular body assembly	IGI: Inferred from Genetic Interaction with SGD:YPT52 Assigned on 2013-05-20	Nickerson DP, et al.  (2012) Termination of isoform-selective Vps21/Rab5 signaling at endolysosomal organelles by Msb3/Gyp3. Traffic 13(10):1411-28	SGD
protein localization to endosome	IGI: Inferred from Genetic Interaction with SGD:YPT52 Assigned on 2013-05-16	Cabrera M, et al.  (2013) Functional separation of endosomal fusion factors and the class C core vacuole/endosome tethering (CORVET) complex in endosome biogenesis. J Biol Chem 288(7):5166-75	SGD
protein targeting to vacuole	IMP: Inferred from Mutant Phenotype Assigned on 2002-11-13	Singer-Kruger B, et al.  (1994) Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast. J Cell Biol 125(2):283-98	SGD
vacuole inheritance	IMP: Inferred from Mutant Phenotype Assigned on 2010-05-21	Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
late endosome	IMP: Inferred from Mutant Phenotype Assigned on 2002-10-03	Prescianotto-Baschong C and Riezman H  (2002) Ordering of compartments in the yeast endocytic pathway. Traffic 3(1):37-49	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrial outer membrane	IDA: Inferred from Direct Assay Assigned on 2006-03-17	Zahedi RP, et al.  (2006) Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins. Mol Biol Cell 17(3):1436-50	SGD
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-09-28	Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
GTP binding	IEA: Inferred from Electronic Annotation with EBI:IPR005225, EBI:IPR001806, EBI:IPR003579 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0342 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein transport	IEA: Inferred from Electronic Annotation with EBI:IPR003579 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
small GTPase mediated signal transduction	IEA: Inferred from Electronic Annotation with EBI:IPR001806, EBI:IPR003579 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
endosome	IEA: Inferred from Electronic Annotation with EBI:KW-0967 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
endosome membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0100 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitochondrial membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0171 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
mitochondrion	IEA: Inferred from Electronic Annotation with EBI:KW-0496 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).