This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2009-09-10    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein binding, bridging	IMP: Inferred from Mutant Phenotype Assigned on 2009-03-02	Araki Y, et al.  (2001) Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast. EMBO J 20(17):4684-93	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
nonfunctional rRNA decay	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-10	Cole SE, et al.  (2009) A convergence of rRNA and mRNA quality control pathways revealed by mechanistic analysis of nonfunctional rRNA decay. Mol Cell 34(4):440-50	SGD
nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay	IGI: Inferred from Genetic Interaction with SGD:DCP1 Assigned on 2009-03-06 IMP: Inferred from Mutant Phenotype Assigned on 2009-03-06	Takahashi S, et al.  (2003) Interaction between Ski7p and Upf1p is required for nonsense-mediated 3'-to-5' mRNA decay in yeast. EMBO J 22(15):3951-9	SGD
	IGI: Inferred from Genetic Interaction with SGD:DCP1 Assigned on 2009-03-06	Mitchell P and Tollervey D  (2003) An NMD pathway in yeast involving accelerated deadenylation and exosome-mediated 3'-->5' degradation. Mol Cell 11(5):1405-13	SGD
nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'	IGI: Inferred from Genetic Interaction with SGD:DCP1, SGD:DCP2 Assigned on 2008-11-07 IMP: Inferred from Mutant Phenotype Assigned on 2008-11-07	van Hoof A, et al.  (2000) Function of the ski4p (Csl4p) and Ski7p proteins in 3'-to-5' degradation of mRNA. Mol Cell Biol 20(21):8230-43	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-03-02	Araki Y, et al.  (2001) Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast. EMBO J 20(17):4684-93	SGD
nuclear-transcribed mRNA catabolic process, non-stop decay	IMP: Inferred from Mutant Phenotype Assigned on 2009-03-06	Wilson MA, et al.  (2007) A genomic screen in yeast reveals novel aspects of nonstop mRNA metabolism. Genetics 177(2):773-84	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-03-06	van Hoof A, et al.  (2002) Exosome-mediated recognition and degradation of mRNAs lacking a termination codon. Science 295(5563):2262-4	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2009-03-02	Araki Y, et al.  (2001) Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast. EMBO J 20(17):4684-93	SGD
colocalizes_with cytoplasmic exosome (RNase complex)	IDA: Inferred from Direct Assay Assigned on 2009-09-10	Synowsky SA, et al.  (2009) Comparative multiplexed mass spectrometric analyses of endogenously expressed yeast nuclear and cytoplasmic exosomes. J Mol Biol 385(4):1300-13	SGD
	IDA: Inferred from Direct Assay Assigned on 2009-03-02	Araki Y, et al.  (2001) Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast. EMBO J 20(17):4684-93	SGD
colocalizes_with Ski complex	IDA: Inferred from Direct Assay Assigned on 2009-03-02	Araki Y, et al.  (2001) Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast. EMBO J 20(17):4684-93	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for SKI7

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
GTP binding	IEA: Inferred from Electronic Annotation with EBI:IPR000795 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0342 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
GTPase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000795 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
GTP catabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR000795 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	GOC
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay	IEA: Inferred from Electronic Annotation with EBI:KW-0866 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
regulation of translation	IEA: Inferred from Electronic Annotation with EBI:KW-0810 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
translation	IEA: Inferred from Electronic Annotation with EBI:KW-0648 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).