This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2004-08-04    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
molecular_function unknown	ND: No Biological Data Available Assigned on 2003-02-03	SGD  (2002) Use of the ND evidence code for Gene Ontology (GO) terms in SGD ()	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
maintenance of meiotic sister chromatid cohesion	IMP: Inferred from Mutant Phenotype Assigned on 2010-07-16	Marston AL, et al.  (2004) A genome-wide screen identifies genes required for centromeric cohesion. Science 303(5662):1367-70	SGD
meiotic sister chromatid segregation	IMP: Inferred from Mutant Phenotype Assigned on 2010-07-08	Katis VL, et al.  (2004) Maintenance of cohesin at centromeres after meiosis I in budding yeast requires a kinetochore-associated protein related to MEI-S332. Curr Biol 14(7):560-72	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2004-02-09	Kitajima TS, et al.  (2004) The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis. Nature 427(6974):510-7	SGD
meiotic sister chromatid separation	IMP: Inferred from Mutant Phenotype Assigned on 2010-07-08	Katis VL, et al.  (2004) Maintenance of cohesin at centromeres after meiosis I in budding yeast requires a kinetochore-associated protein related to MEI-S332. Curr Biol 14(7):560-72	SGD
mitotic sister chromatid segregation	IGI: Inferred from Genetic Interaction with SGD:MPS1 Assigned on 2010-07-08 IMP: Inferred from Mutant Phenotype Assigned on 2005-02-25	Indjeian VB, et al.  (2005) The centromeric protein Sgo1 is required to sense lack of tension on mitotic chromosomes. Science 307(5706):130-3	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-07-08	Katis VL, et al.  (2004) Maintenance of cohesin at centromeres after meiosis I in budding yeast requires a kinetochore-associated protein related to MEI-S332. Curr Biol 14(7):560-72	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2004-02-09	Kitajima TS, et al.  (2004) The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis. Nature 427(6974):510-7	SGD
mitotic spindle assembly checkpoint	IGI: Inferred from Genetic Interaction with SGD:MCD1, SGD:CDC28, SGD:MPS1 Assigned on 2005-11-14 IMP: Inferred from Mutant Phenotype Assigned on 2005-11-14	Indjeian VB, et al.  (2005) The centromeric protein Sgo1 is required to sense lack of tension on mitotic chromosomes. Science 307(5706):130-3	SGD
positive regulation of maintenance of meiotic sister chromatid cohesion	IGI: Inferred from Genetic Interaction with SGD:MAM1 Assigned on 2010-07-08 IMP: Inferred from Mutant Phenotype Assigned on 2010-07-08	Katis VL, et al.  (2004) Maintenance of cohesin at centromeres after meiosis I in budding yeast requires a kinetochore-associated protein related to MEI-S332. Curr Biol 14(7):560-72	SGD
sister chromatid biorientation	IMP: Inferred from Mutant Phenotype Assigned on 2008-09-18	Fernius J and Hardwick KG  (2007) Bub1 kinase targets Sgo1 to ensure efficient chromosome biorientation in budding yeast mitosis. PLoS Genet 3(11):e213	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2008-09-29	Indjeian VB and Murray AW  (2007) Budding yeast mitotic chromosomes have an intrinsic bias to biorient on the spindle. Curr Biol 17(21):1837-46	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
condensed nuclear chromosome kinetochore	IDA: Inferred from Direct Assay Assigned on 2009-12-16	Katis VL, et al.  (2004) Maintenance of cohesin at centromeres after meiosis I in budding yeast requires a kinetochore-associated protein related to MEI-S332. Curr Biol 14(7):560-72	SGD
	IDA: Inferred from Direct Assay Assigned on 2009-12-16	Kitajima TS, et al.  (2004) The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis. Nature 427(6974):510-7	SGD
condensed nuclear chromosome, centromeric region	IDA: Inferred from Direct Assay Assigned on 2010-07-15	Kiburz BM, et al.  (2005) The core centromere and Sgo1 establish a 50-kb cohesin-protected domain around centromeres during meiosis I. Genes Dev 19(24):3017-30	SGD
	IDA: Inferred from Direct Assay Assigned on 2009-12-16	Kitajima TS, et al.  (2004) The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis. Nature 427(6974):510-7	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
colocalizes_with spindle pole body	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cell division	IEA: Inferred from Electronic Annotation with EBI:KW-0132 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
chromosome segregation	IEA: Inferred from Electronic Annotation with EBI:KW-0159 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
meiosis	IEA: Inferred from Electronic Annotation with EBI:KW-0469 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
meiotic chromosome segregation	IEA: Inferred from Electronic Annotation with EBI:IPR011515 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
mitosis	IEA: Inferred from Electronic Annotation with EBI:KW-0498 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
chromosome	IEA: Inferred from Electronic Annotation with EBI:KW-0158 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
chromosome, centromeric region	IEA: Inferred from Electronic Annotation with EBI:IPR011515 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0047 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0137 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
condensed chromosome kinetochore	IEA: Inferred from Electronic Annotation with EBI:SL-0149 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cytoskeleton	IEA: Inferred from Electronic Annotation with EBI:KW-0206 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
kinetochore	IEA: Inferred from Electronic Annotation with EBI:KW-0995 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:IPR011515 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
spindle pole	IEA: Inferred from Electronic Annotation with EBI:SL-0448 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).