This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-01-16    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
transmembrane signaling receptor activity	IGI: Inferred from Genetic Interaction Assigned on 2002-12-06 ISS: Inferred from Sequence or structural Similarity Assigned on 2002-12-06	Lodder AL, et al.  (1999) Characterization of the Wsc1 protein, a putative receptor in the stress response of Saccharomyces cerevisiae. Genetics 152(4):1487-99	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
actin cytoskeleton organization	IMP: Inferred from Mutant Phenotype Assigned on 2003-01-16	Delley PA and Hall MN  (1999) Cell wall stress depolarizes cell growth via hyperactivation of RHO1. J Cell Biol 147(1):163-74	SGD
establishment of cell polarity	IMP: Inferred from Mutant Phenotype Assigned on 2001-03-02	Delley PA and Hall MN  (1999) Cell wall stress depolarizes cell growth via hyperactivation of RHO1. J Cell Biol 147(1):163-74	SGD
fungal-type cell wall organization	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Ketela T, et al.  (1999) Saccharomyces cerevisiae mid2p is a potential cell wall stress sensor and upstream activator of the PKC1-MPK1 cell integrity pathway. J Bacteriol 181(11):3330-40	SGD
peroxisome degradation	IMP: Inferred from Mutant Phenotype Assigned on 2010-05-04	Manjithaya R, et al.  (2010) A yeast MAPK cascade regulates pexophagy but not other autophagy pathways. J Cell Biol 189(2):303-10	SGD
positive regulation of endocytosis	IMP: Inferred from Mutant Phenotype Assigned on 2009-11-13	deHart AK, et al.  (2003) Receptor internalization in yeast requires the Tor2-Rho1 signaling pathway. Mol Biol Cell 14(11):4676-84	SGD
response to heat	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Ketela T, et al.  (1999) Saccharomyces cerevisiae mid2p is a potential cell wall stress sensor and upstream activator of the PKC1-MPK1 cell integrity pathway. J Bacteriol 181(11):3330-40	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Delley PA and Hall MN  (1999) Cell wall stress depolarizes cell growth via hyperactivation of RHO1. J Cell Biol 147(1):163-74	SGD
response to osmotic stress	IGI: Inferred from Genetic Interaction with SGD:PPZ1 Assigned on 2005-05-05	Merchan S, et al.  (2004) Response of the Saccharomyces cerevisiae Mpk1 mitogen-activated protein kinase pathway to increases in internal turgor pressure caused by loss of Ppz protein phosphatases. Eukaryot Cell 3(1):100-7	SGD
Rho protein signal transduction	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Ketela T, et al.  (1999) Saccharomyces cerevisiae mid2p is a potential cell wall stress sensor and upstream activator of the PKC1-MPK1 cell integrity pathway. J Bacteriol 181(11):3330-40	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Delley PA and Hall MN  (1999) Cell wall stress depolarizes cell growth via hyperactivation of RHO1. J Cell Biol 147(1):163-74	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular bud neck	IDA: Inferred from Direct Assay Assigned on 2008-10-21	Straede A and Heinisch JJ  (2007) Functional analyses of the extra- and intracellular domains of the yeast cell wall integrity sensors Mid2 and Wsc1. FEBS Lett 581(23):4495-500	SGD
plasma membrane	IDA: Inferred from Direct Assay Assigned on 2005-06-30	Verna J, et al.  (1997) A family of genes required for maintenance of cell wall integrity and for the stress response in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 94(25):13804-9	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mating projection tip	IDA: Inferred from Direct Assay Assigned on 2008-12-19	Narayanaswamy R, et al.  (2009) Systematic Definition of Protein Constituents along the Major Polarization Axis Reveals an Adaptive Reuse of the Polarization Machinery in Pheromone-Treated Budding Yeast. J Proteome Res 8(1):6-19	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
response to stress	IEA: Inferred from Electronic Annotation with EBI:KW-0346 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0812 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
plasma membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0039 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-1003 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).