This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2006-09-12    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA topoisomerase type I activity	IMP: Inferred from Mutant Phenotype Assigned on 2006-09-12	Thrash C, et al.  (1985) Cloning, characterization, and sequence of the yeast DNA topoisomerase I gene. Proc Natl Acad Sci U S A 82(13):4374-8	SGD
	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Goto T, et al.  (1984) The purification and characterization of DNA topoisomerases I and II of the yeast Saccharomyces cerevisiae. J Biol Chem 259(16):10422-9	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin assembly or disassembly	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Garinther WI and Schultz MC  (1997) Topoisomerase function during replication-independent chromatin assembly in yeast. Mol Cell Biol 17(7):3520-6	SGD
chromatin silencing at rDNA	IMP: Inferred from Mutant Phenotype Assigned on 2006-09-08	Smith JS, et al.  (1999) A genetic screen for ribosomal DNA silencing defects identifies multiple DNA replication and chromatin-modulating factors. Mol Cell Biol 19(4):3184-97	SGD
DNA strand elongation involved in DNA replication	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Kim RA and Wang JC  (1989) Function of DNA topoisomerases as replication swivels in Saccharomyces cerevisiae. J Mol Biol 208(2):257-67	SGD
DNA topological change	IMP: Inferred from Mutant Phenotype Assigned on 2006-09-12	Thrash C, et al.  (1985) Cloning, characterization, and sequence of the yeast DNA topoisomerase I gene. Proc Natl Acad Sci U S A 82(13):4374-8	SGD
	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Goto T, et al.  (1984) The purification and characterization of DNA topoisomerases I and II of the yeast Saccharomyces cerevisiae. J Biol Chem 259(16):10422-9	SGD
mitotic chromosome condensation	IGI: Inferred from Genetic Interaction Assigned on 2001-01-18 IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Castano IB, et al.  (1996) Mitotic chromosome condensation in the rDNA requires TRF4 and DNA topoisomerase I in Saccharomyces cerevisiae. Genes Dev 10(20):2564-76	SGD
nuclear migration	IGI: Inferred from Genetic Interaction Assigned on 2001-01-18 IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Castano IB, et al.  (1996) Mitotic chromosome condensation in the rDNA requires TRF4 and DNA topoisomerase I in Saccharomyces cerevisiae. Genes Dev 10(20):2564-76	SGD
regulation of mitotic recombination	IMP: Inferred from Mutant Phenotype Assigned on 2003-08-14	Christman MF, et al.  (1988) Mitotic recombination in the rDNA of S. cerevisiae is suppressed by the combined action of DNA topoisomerases I and II. Cell 55(3):413-25	SGD
regulation of transcription from RNA polymerase II promoter	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Choder M  (1991) A general topoisomerase I-dependent transcriptional repression in the stationary phase in yeast. Genes Dev 5(12A):2315-26	SGD
transcription elongation from RNA polymerase II promoter	IMP: Inferred from Mutant Phenotype Assigned on 2006-09-12	Brill SJ and Sternglanz R  (1988) Transcription-dependent DNA supercoiling in yeast DNA topoisomerase mutants. Cell 54(3):403-11	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleolus	IPI: Inferred from Physical Interaction with SGD:NSR1 Assigned on 2006-11-27	Edwards TK, et al.  (2000) Role for nucleolin/Nsr1 in the cellular localization of topoisomerase I. J Biol Chem 275(46):36181-8	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Goto T, et al.  (1984) The purification and characterization of DNA topoisomerases I and II of the yeast Saccharomyces cerevisiae. J Biol Chem 259(16):10422-9	SGD
replication fork protection complex	IDA: Inferred from Direct Assay Assigned on 2009-08-24	Gambus A, et al.  (2006) GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks. Nat Cell Biol 8(4):358-66	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for TOP1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
chromatin DNA binding	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10290_AN0 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
DNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR013030, EBI:IPR008336, EBI:IPR001631, EBI:IPR018521, EBI:IPR014727, EBI:IPR013500, EBI:IPR014711, EBI:IPR011010, EBI:IPR013499 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DNA topoisomerase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0799 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DNA topoisomerase type I activity	IEA: Inferred from Electronic Annotation with EBI:IPR013030, EBI:IPR008336, EBI:IPR001631, EBI:IPR018521, EBI:IPR014727, EBI:IPR014711, EBI:IPR013034, EBI:IPR013499 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:5.99.1.2 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
DNA topoisomerase type II (ATP-hydrolyzing) activity	IEA: Inferred from Electronic Annotation with EBI:IPR013500 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
isomerase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0413 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA topological change	IEA: Inferred from Electronic Annotation with EBI:IPR014711, EBI:IPR013499, EBI:IPR013030, EBI:IPR008336, EBI:IPR001631, EBI:IPR013500, EBI:IPR018521, EBI:IPR014727 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
chromosome	IEA: Inferred from Electronic Annotation with EBI:IPR014711, EBI:IPR013499, EBI:IPR013030, EBI:IPR008336, EBI:IPR001631, EBI:IPR014727, EBI:IPR013500 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleolus	IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0190 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).