This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2002-05-07    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
citrate (Si)-synthase activity	IMP: Inferred from Mutant Phenotype Assigned on 2007-06-25 IDA: Inferred from Direct Assay Assigned on 2007-06-25	Graybill ER, et al.  (2007) Functional comparison of citrate synthase isoforms from S. cerevisiae. Arch Biochem Biophys 465(1):26-37	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
acetyl-CoA catabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2007-06-25 IDA: Inferred from Direct Assay Assigned on 2007-06-25	Graybill ER, et al.  (2007) Functional comparison of citrate synthase isoforms from S. cerevisiae. Arch Biochem Biophys 465(1):26-37	SGD
citrate metabolic process	TAS: Traceable Author Statement Assigned on 2001-01-18	McAlister-Henn L and Small WC  (1997) Molecular genetics of yeast TCA cycle isozymes. Prog Nucleic Acid Res Mol Biol 57:317-39	SGD
glutamate biosynthetic process	TAS: Traceable Author Statement Assigned on 2001-01-18	McAlister-Henn L and Small WC  (1997) Molecular genetics of yeast TCA cycle isozymes. Prog Nucleic Acid Res Mol Biol 57:317-39	SGD
tricarboxylic acid cycle	TAS: Traceable Author Statement Assigned on 2001-01-18	McAlister-Henn L and Small WC  (1997) Molecular genetics of yeast TCA cycle isozymes. Prog Nucleic Acid Res Mol Biol 57:317-39	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2009-07-01	Grandier-Vazeille X, et al.  (2001) Yeast mitochondrial dehydrogenases are associated in a supramolecular complex. Biochemistry 40(33):9758-69	SGD
	IPI: Inferred from Physical Interaction with SGD:ENO2, SGD:ENO1 Assigned on 2007-03-05	Brandina I, et al.  (2006) Enolase takes part in a macromolecular complex associated to mitochondria in yeast. Biochim Biophys Acta 1757(9-10):1217-1228	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2002-05-07	Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19	SGD
	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-09-24	Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
citrate (Si)-synthase activity	IEA: Inferred from Electronic Annotation with EBI:IPR010109 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:2.3.3.1 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
transferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer	IEA: Inferred from Electronic Annotation with EBI:IPR016142, EBI:IPR002020, EBI:IPR019810, EBI:IPR016141 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular carbohydrate metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR016142, EBI:IPR010109, EBI:IPR002020, EBI:IPR016141 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
tricarboxylic acid cycle	IEA: Inferred from Electronic Annotation with EBI:IPR019810 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with UniPathway:UPA00223 Last updated 2013-03-02	UniProt-GOA  (2012) Gene Ontology annotation based on UniPathway vocabulary mapping.	UniPathway
	IEA: Inferred from Electronic Annotation with EBI:KW-0816 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrial matrix	IEA: Inferred from Electronic Annotation with EBI:SL-0170 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
mitochondrion	IEA: Inferred from Electronic Annotation with EBI:KW-0496 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).