This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2009-07-23    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
molecular_function unknown	ND: No Biological Data Available Assigned on 2009-07-23	SGD  (2002) Use of the ND evidence code for Gene Ontology (GO) terms in SGD ()	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA repair	IGI: Inferred from Genetic Interaction with SGD:RAD9 Assigned on 2009-07-13 IMP: Inferred from Mutant Phenotype Assigned on 2009-07-13	Redon C, et al.  (2006) Genetic analysis of Saccharomyces cerevisiae H2A serine 129 mutant suggests a functional relationship between H2A and the sister-chromatid cohesion partners Csm3-Tof1 for the repair of topoisomerase I-induced DNA damage. Genetics 172(1):67-76	SGD
DNA replication	IMP: Inferred from Mutant Phenotype Assigned on 2009-07-10	Tourriere H, et al.  (2005) Mrc1 and Tof1 promote replication fork progression and recovery independently of Rad53. Mol Cell 19(5):699-706	SGD
DNA replication checkpoint	IMP: Inferred from Mutant Phenotype Assigned on 2004-02-10 IPI: Inferred from Physical Interaction Assigned on 2004-02-10	Katou Y, et al.  (2003) S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex. Nature 424(6952):1078-83	SGD
maintenance of DNA repeat elements	IMP: Inferred from Mutant Phenotype Assigned on 2009-07-23	Razidlo DF and Lahue RS  (2008) Mrc1, Tof1 and Csm3 inhibit CAG.CTG repeat instability by at least two mechanisms. DNA Repair (Amst) 7(4):633-40	SGD
mitotic sister chromatid cohesion	IGI: Inferred from Genetic Interaction with SGD:CTF8 Assigned on 2005-05-13 IMP: Inferred from Mutant Phenotype Assigned on 2005-05-13	Mayer ML, et al.  (2004) Identification of protein complexes required for efficient sister chromatid cohesion. Mol Biol Cell 15(4):1736-45	SGD
	IGI: Inferred from Genetic Interaction with SGD:MRC1, SGD:CTF8 Assigned on 2008-09-18 IMP: Inferred from Mutant Phenotype Assigned on 2008-09-18	Xu H, et al.  (2007) Genetic dissection of parallel sister-chromatid cohesion pathways. Genetics 176(3):1417-29	SGD
replication fork arrest	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-03	Hodgson B, et al.  (2007) Mrc1 and Tof1 Regulate DNA Replication Forks in Different Ways during Normal S Phase. Mol Biol Cell 18(10):3894-902	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-07-21	Mohanty BK, et al.  (2006) The Tof1p-Csm3p protein complex counteracts the Rrm3p helicase to control replication termination of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 103(4):897-902	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-07-10	Calzada A, et al.  (2005) Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork. Genes Dev 19(16):1905-19	SGD
replication fork protection	IMP: Inferred from Mutant Phenotype Assigned on 2009-07-21	Mohanty BK, et al.  (2006) The Tof1p-Csm3p protein complex counteracts the Rrm3p helicase to control replication termination of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 103(4):897-902	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2008-08-04	Voineagu I, et al.  (2008) Replication stalling at unstable inverted repeats: interplay between DNA hairpins and fork stabilizing proteins. Proc Natl Acad Sci U S A 105(29):9936-41	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nuclear chromosome	IPI: Inferred from Physical Interaction Assigned on 2004-02-10	Katou Y, et al.  (2003) S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex. Nature 424(6952):1078-83	SGD
nuclear replication fork	IDA: Inferred from Direct Assay Assigned on 2009-07-10	Calzada A, et al.  (2005) Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork. Genes Dev 19(16):1905-19	SGD
replication fork protection complex	IDA: Inferred from Direct Assay Assigned on 2009-08-24	Gambus A, et al.  (2006) GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks. Nat Cell Biol 8(4):358-66	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for TOF1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DNA repair	IEA: Inferred from Electronic Annotation with EBI:KW-0234 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
meiosis	IEA: Inferred from Electronic Annotation with EBI:KW-0469 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
negative regulation of DNA replication	IEA: Inferred from Electronic Annotation with EBI:KW-0236 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
response to DNA damage stimulus	IEA: Inferred from Electronic Annotation with EBI:KW-0227 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).