This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-02-09    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
mRNA binding	IDA: Inferred from Direct Assay Assigned on 2011-02-04	Scherrer T, et al.  (2011) Defining potentially conserved RNA regulons of homologous zinc-finger RNA-binding proteins. Genome Biol 12(1):R3	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-02-01	Tsvetanova NG, et al.  (2010) Proteome-Wide Search Reveals Unexpected RNA-Binding Proteins in Saccharomyces cerevisiae.LID - e12671 [pii] PLoS One 5(9)	SGD
single-stranded DNA binding	IDA: Inferred from Direct Assay Assigned on 2011-02-04	Scherrer T, et al.  (2011) Defining potentially conserved RNA regulons of homologous zinc-finger RNA-binding proteins. Genome Biol 12(1):R3	SGD
translation regulator activity	IDA: Inferred from Direct Assay Assigned on 2011-02-09	Sammons MA, et al.  (2011) Saccharomyces cerevisiae Gis2 interacts with the translation machinery and is orthogonal to myotonic dystrophy type 2 protein ZNF9. Biochem Biophys Res Commun 406(1):13-9	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
positive regulation of translation	IDA: Inferred from Direct Assay Assigned on 2011-02-09	Sammons MA, et al.  (2011) Saccharomyces cerevisiae Gis2 interacts with the translation machinery and is orthogonal to myotonic dystrophy type 2 protein ZNF9. Biochem Biophys Res Commun 406(1):13-9	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasmic mRNA processing body	IDA: Inferred from Direct Assay Assigned on 2013-01-09	Rojas M, et al.  (2012) Yeast Gis2 and Its Human Ortholog CNBP Are Novel Components of Stress-Induced RNP Granules. PLoS One 7(12):e52824	SGD
cytoplasmic stress granule	IDA: Inferred from Direct Assay Assigned on 2013-01-09	Rojas M, et al.  (2012) Yeast Gis2 and Its Human Ortholog CNBP Are Novel Components of Stress-Induced RNP Granules. PLoS One 7(12):e52824	SGD
polysomal ribosome	IPI: Inferred from Physical Interaction Assigned on 2011-02-09	Sammons MA, et al.  (2011) Saccharomyces cerevisiae Gis2 interacts with the translation machinery and is orthogonal to myotonic dystrophy type 2 protein ZNF9. Biochem Biophys Res Commun 406(1):13-9	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleic acid binding	IEA: Inferred from Electronic Annotation with EBI:IPR001878 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
zinc ion binding	IEA: Inferred from Electronic Annotation with EBI:IPR001878 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).