This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2012-05-10    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein domain specific binding	IDA: Inferred from Direct Assay Assigned on 2012-05-10	Vasiljeva L, et al.  (2008) The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain. Nat Struct Mol Biol 15(8):795-804	SGD
RNA binding	IDA: Inferred from Direct Assay Assigned on 2002-03-04	Steinmetz EJ and Brow DA  (1998) Control of pre-mRNA accumulation by the essential yeast protein Nrd1 requires high-affinity transcript binding and a domain implicated in RNA polymerase II association. Proc Natl Acad Sci U S A 95(12):6699-704	SGD
transcription regulatory region RNA binding	IDA: Inferred from Direct Assay Assigned on 2011-01-21	Hobor F, et al.  (2011) Recognition of Transcription Termination Signal by the Nuclear Polyadenylated RNA-binding (NAB) 3 Protein. J Biol Chem 286(5):3645-57	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
antisense RNA transcript catabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2012-05-10	Creamer TJ, et al.  (2011) Transcriptome-Wide Binding Sites for Components of the Saccharomyces cerevisiae Non-Poly(A) Termination Pathway: Nrd1, Nab3, and Sen1. PLoS Genet 7(10):e1002329	SGD
CUT catabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2012-05-10	Arigo JT, et al.  (2006) Termination of cryptic unstable transcripts is directed by yeast RNA-binding proteins Nrd1 and Nab3. Mol Cell 23(6):841-51	SGD
mRNA 3'-end processing	IMP: Inferred from Mutant Phenotype Assigned on 2012-05-10	Steinmetz EJ, et al.  (2001) RNA-binding protein Nrd1 directs poly(A)-independent 3'-end formation of RNA polymerase II transcripts. Nature 413(6853):327-31	SGD
nuclear mRNA surveillance	IMP: Inferred from Mutant Phenotype Assigned on 2012-05-10	Honorine R, et al.  (2011) Nuclear mRNA quality control in yeast is mediated by Nrd1 co-transcriptional recruitment, as revealed by the targeting of Rho-induced aberrant transcripts. Nucleic Acids Res 39(7):2809-20	SGD
snoRNA 3'-end processing	IMP: Inferred from Mutant Phenotype Assigned on 2012-05-10	Steinmetz EJ, et al.  (2001) RNA-binding protein Nrd1 directs poly(A)-independent 3'-end formation of RNA polymerase II transcripts. Nature 413(6853):327-31	SGD
snRNA 3'-end processing	IMP: Inferred from Mutant Phenotype Assigned on 2012-05-10	Steinmetz EJ, et al.  (2001) RNA-binding protein Nrd1 directs poly(A)-independent 3'-end formation of RNA polymerase II transcripts. Nature 413(6853):327-31	SGD
termination of RNA polymerase II transcription, exosome-dependent	IDA: Inferred from Direct Assay Assigned on 2012-05-10	Vasiljeva L and Buratowski S  (2006) Nrd1 interacts with the nuclear exosome for 3' processing of RNA polymerase II transcripts. Mol Cell 21(2):239-48	SGD
tRNA 3'-end processing	IMP: Inferred from Mutant Phenotype Assigned on 2012-05-10	Wlotzka W, et al.  (2011) The nuclear RNA polymerase II surveillance system targets polymerase III transcripts. EMBO J 30(9):1790-803	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
Nrd1 complex	IDA: Inferred from Direct Assay Assigned on 2011-01-30	Conrad NK, et al.  (2000) A yeast heterogeneous nuclear ribonucleoprotein complex associated with RNA polymerase II. Genetics 154(2):557-71	SGD
nucleus	IC: Inferred By Curator from transcription regulatory region RNA binding Assigned on 2012-05-10	Hobor F, et al.  (2011) Recognition of Transcription Termination Signal by the Nuclear Polyadenylated RNA-binding (NAB) 3 Protein. J Biol Chem 286(5):3645-57	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for NRD1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleic acid binding	IEA: Inferred from Electronic Annotation with EBI:IPR000504 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:IPR012677 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
RNA binding	IEA: Inferred from Electronic Annotation with EBI:KW-0694 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).