This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2004-11-12    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein binding	IPI: Inferred from Physical Interaction with SGD:GLC7 Assigned on 2011-03-02	Kozubowski L, et al.  (2003) A Bni4-Glc7 phosphatase complex that recruits chitin synthase to the site of bud emergence. Mol Biol Cell 14(1):26-39	SGD
	IPI: Inferred from Physical Interaction with SGD:CDC10, SGD:SKT5 Assigned on 2011-03-01	DeMarini DJ, et al.  (1997) A septin-based hierarchy of proteins required for localized deposition of chitin in the Saccharomyces cerevisiae cell wall. J Cell Biol 139(1):75-93	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
asymmetric protein localization	IMP: Inferred from Mutant Phenotype Assigned on 2005-09-20 IDA: Inferred from Direct Assay Assigned on 2005-09-20	Kozubowski L, et al.  (2003) A Bni4-Glc7 phosphatase complex that recruits chitin synthase to the site of bud emergence. Mol Biol Cell 14(1):26-39	SGD
barrier septum assembly	IGI: Inferred from Genetic Interaction Assigned on 2004-11-12	Sanz M, et al.  (2004) Saccharomyces cerevisiae Bni4p directs the formation of the chitin ring and also participates in the correct assembly of the septum structure. Microbiology 150(Pt 10):3229-41	SGD
chitin biosynthetic process	IGI: Inferred from Genetic Interaction Assigned on 2004-11-12 IMP: Inferred from Mutant Phenotype Assigned on 2004-11-12 TAS: Traceable Author Statement Assigned on 2004-11-12	Sanz M, et al.  (2004) Saccharomyces cerevisiae Bni4p directs the formation of the chitin ring and also participates in the correct assembly of the septum structure. Microbiology 150(Pt 10):3229-41	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular bud neck	IDA: Inferred from Direct Assay Assigned on 2004-10-08	Sundin BA, et al.  (2004) Localization of proteins that are coordinately expressed with Cln2 during the cell cycle. Yeast 21(9):793-800	SGD
cellular bud neck septin collar	IDA: Inferred from Direct Assay Assigned on 2009-11-04	Kozubowski L, et al.  (2005) Role of the septin ring in the asymmetric localization of proteins at the mother-bud neck in Saccharomyces cerevisiae. Mol Biol Cell 16(8):3455-66	SGD
incipient cellular bud site	IDA: Inferred from Direct Assay Assigned on 2009-11-04	Kozubowski L, et al.  (2005) Role of the septin ring in the asymmetric localization of proteins at the mother-bud neck in Saccharomyces cerevisiae. Mol Biol Cell 16(8):3455-66	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-11-12	Sanz M, et al.  (2004) Saccharomyces cerevisiae Bni4p directs the formation of the chitin ring and also participates in the correct assembly of the septum structure. Microbiology 150(Pt 10):3229-41	SGD
septin ring	IDA: Inferred from Direct Assay Assigned on 2009-11-04	Kozubowski L, et al.  (2005) Role of the septin ring in the asymmetric localization of proteins at the mother-bud neck in Saccharomyces cerevisiae. Mol Biol Cell 16(8):3455-66	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-11-12 IPI: Inferred from Physical Interaction Assigned on 2004-11-12	DeMarini DJ, et al.  (1997) A septin-based hierarchy of proteins required for localized deposition of chitin in the Saccharomyces cerevisiae cell wall. J Cell Biol 139(1):75-93	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for BNI4

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

There are no Computational annotations for BNI4

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).