This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-09-23    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA-(apurinic or apyrimidinic site) lyase activity	IDA: Inferred from Direct Assay Assigned on 2008-09-23	Jung SO, et al.  (2001) Yeast ribosomal protein S3 has an endonuclease activity on AP DNA. Mol Cells 12(1):84-90	SGD
structural constituent of ribosome	IDA: Inferred from Direct Assay Assigned on 2008-09-15	Otaka E, et al.  (1984) Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes. Mol Gen Genet 195(3):544-6	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasmic translation	IC: Inferred By Curator from cytosolic small ribosomal subunit Assigned on 2008-09-23	Higo K and Otaka E  (1979) Isolation and characterization of fourteen ribosomal proteins from small subunits of yeast. Biochemistry 18(19):4191-6	SGD
	IC: Inferred By Curator from cytosolic small ribosomal subunit Assigned on 2008-09-23	Yeh YC, et al.  (1986) Protein topography of the 40 S ribosomal subunit from Saccharomyces cerevisiae as shown by chemical cross-linking. J Biol Chem 261(30):14148-53	SGD
	IC: Inferred By Curator from cytosolic small ribosomal subunit Assigned on 2008-09-23	Otaka E, et al.  (1984) Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes. Mol Gen Genet 195(3):544-6	SGD
ribosomal small subunit export from nucleus	IGI: Inferred from Genetic Interaction with SGD:YAR1 Assigned on 2012-11-02	Koch B, et al.  (2012) Yar1 protects the ribosomal protein Rps3 from aggregation. J Biol Chem 287(26):21806-15	SGD
rRNA export from nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2008-09-23	Ferreira-Cerca S, et al.  (2005) Roles of eukaryotic ribosomal proteins in maturation and transport of pre-18S rRNA and ribosome function. Mol Cell 20(2):263-75	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytosolic small ribosomal subunit	IDA: Inferred from Direct Assay Assigned on 2008-09-23	Higo K and Otaka E  (1979) Isolation and characterization of fourteen ribosomal proteins from small subunits of yeast. Biochemistry 18(19):4191-6	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-09-23	Yeh YC, et al.  (1986) Protein topography of the 40 S ribosomal subunit from Saccharomyces cerevisiae as shown by chemical cross-linking. J Biol Chem 261(30):14148-53	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-09-15	Otaka E, et al.  (1984) Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes. Mol Gen Genet 195(3):544-6	SGD
preribosome, small subunit precursor	IDA: Inferred from Direct Assay Assigned on 2006-11-27	Schafer T, et al.  (2006) Hrr25-dependent phosphorylation state regulates organization of the pre-40S subunit. Nature 441(7093):651-5	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
90S preribosome	IDA: Inferred from Direct Assay Assigned on 2012-02-08	Grandi P, et al.  (2002) 90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors. Mol Cell 10(1):105-15	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
RNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR009019, EBI:IPR004044, EBI:IPR004087, EBI:IPR018280 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0694 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
structural constituent of ribosome	IEA: Inferred from Electronic Annotation with EBI:IPR018280, EBI:IPR001351, EBI:IPR005703 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
translation	IEA: Inferred from Electronic Annotation with EBI:IPR018280, EBI:IPR001351, EBI:IPR005703 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
intracellular	IEA: Inferred from Electronic Annotation with EBI:IPR018280, EBI:IPR001351 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
ribonucleoprotein complex	IEA: Inferred from Electronic Annotation with EBI:KW-0687 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
ribosome	IEA: Inferred from Electronic Annotation with EBI:IPR018280, EBI:IPR001351 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0689 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
small ribosomal subunit	IEA: Inferred from Electronic Annotation with EBI:IPR005703 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).