This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2011-12-16    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
mitogen-activated protein kinase binding	IDA: Inferred from Direct Assay Assigned on 2011-12-12	Proft M, et al.  (2001) Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase in response to osmotic stress. EMBO J 20(5):1123-33	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-12	Proft M and Struhl K  (2002) Hog1 kinase converts the Sko1-Cyc8-Tup1 repressor complex into an activator that recruits SAGA and SWI/SNF in response to osmotic stress. Mol Cell 9(6):1307-17	SGD
RNA polymerase II core promoter proximal region sequence-specific DNA binding	IDA: Inferred from Direct Assay Assigned on 2011-12-12	Nehlin JO, et al.  (1992) Yeast SKO1 gene encodes a bZIP protein that binds to the CRE motif and acts as a repressor of transcription. Nucleic Acids Res 20(20):5271-8	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-12 IDA: Inferred from Direct Assay Assigned on 2011-12-12	Pascual-Ahuir A, et al.  (2001) The Sko1p repressor and Gcn4p activator antagonistically modulate stress-regulated transcription in Saccharomyces cerevisiae. Mol Cell Biol 21(1):16-25	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-12-12	Proft M and Serrano R  (1999) Repressors and upstream repressing sequences of the stress-regulated ENA1 gene in Saccharomyces cerevisiae: bZIP protein Sko1p confers HOG-dependent osmotic regulation. Mol Cell Biol 19(1):537-46	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-12-12	Pascual-Ahuir A, et al.  (2001) Multiple levels of control regulate the yeast cAMP-response element-binding protein repressor Sko1p in response to stress. J Biol Chem 276(40):37373-8	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-12-12	Proft M and Struhl K  (2002) Hog1 kinase converts the Sko1-Cyc8-Tup1 repressor complex into an activator that recruits SAGA and SWI/SNF in response to osmotic stress. Mol Cell 9(6):1307-17	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-12-12	Vincent AC and Struhl K  (1992) ACR1, a yeast ATF/CREB repressor. Mol Cell Biol 12(12):5394-405	SGD
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-12 IDA: Inferred from Direct Assay Assigned on 2011-12-12	Nehlin JO, et al.  (1992) Yeast SKO1 gene encodes a bZIP protein that binds to the CRE motif and acts as a repressor of transcription. Nucleic Acids Res 20(20):5271-8	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-12 IDA: Inferred from Direct Assay Assigned on 2011-12-12	Pascual-Ahuir A, et al.  (2001) The Sko1p repressor and Gcn4p activator antagonistically modulate stress-regulated transcription in Saccharomyces cerevisiae. Mol Cell Biol 21(1):16-25	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-12 IDA: Inferred from Direct Assay Assigned on 2011-12-12	Proft M and Serrano R  (1999) Repressors and upstream repressing sequences of the stress-regulated ENA1 gene in Saccharomyces cerevisiae: bZIP protein Sko1p confers HOG-dependent osmotic regulation. Mol Cell Biol 19(1):537-46	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-12 IDA: Inferred from Direct Assay Assigned on 2011-12-12	Vincent AC and Struhl K  (1992) ACR1, a yeast ATF/CREB repressor. Mol Cell Biol 12(12):5394-405	SGD
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-12 IDA: Inferred from Direct Assay Assigned on 2011-12-12	Proft M and Struhl K  (2002) Hog1 kinase converts the Sko1-Cyc8-Tup1 repressor complex into an activator that recruits SAGA and SWI/SNF in response to osmotic stress. Mol Cell 9(6):1307-17	SGD
RNA polymerase II repressing transcription factor binding	IDA: Inferred from Direct Assay Assigned on 2011-12-12	Proft M, et al.  (2001) Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase in response to osmotic stress. EMBO J 20(5):1123-33	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-12-12	Pascual-Ahuir A, et al.  (2001) Multiple levels of control regulate the yeast cAMP-response element-binding protein repressor Sko1p in response to stress. J Biol Chem 276(40):37373-8	SGD
RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription	IGI: Inferred from Genetic Interaction with SGD:HOG1 Assigned on 2011-12-16 IMP: Inferred from Mutant Phenotype Assigned on 2011-12-16	Proft M, et al.  (2001) Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase in response to osmotic stress. EMBO J 20(5):1123-33	SGD
sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-12	Proft M and Struhl K  (2002) Hog1 kinase converts the Sko1-Cyc8-Tup1 repressor complex into an activator that recruits SAGA and SWI/SNF in response to osmotic stress. Mol Cell 9(6):1307-17	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
negative regulation of transcription from RNA polymerase II promoter	IMP: Inferred from Mutant Phenotype Assigned on 2002-11-15	Nehlin JO, et al.  (1992) Yeast SKO1 gene encodes a bZIP protein that binds to the CRE motif and acts as a repressor of transcription. Nucleic Acids Res 20(20):5271-8	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-12	Pascual-Ahuir A, et al.  (2001) The Sko1p repressor and Gcn4p activator antagonistically modulate stress-regulated transcription in Saccharomyces cerevisiae. Mol Cell Biol 21(1):16-25	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-12	Proft M, et al.  (2001) Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase in response to osmotic stress. EMBO J 20(5):1123-33	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-12	Proft M and Serrano R  (1999) Repressors and upstream repressing sequences of the stress-regulated ENA1 gene in Saccharomyces cerevisiae: bZIP protein Sko1p confers HOG-dependent osmotic regulation. Mol Cell Biol 19(1):537-46	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-12	Garcia-Gimeno MA and Struhl K  (2000) Aca1 and Aca2, ATF/CREB activators in Saccharomyces cerevisiae, are important for carbon source utilization but not the response to stress. Mol Cell Biol 20(12):4340-9	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-12	Vincent AC and Struhl K  (1992) ACR1, a yeast ATF/CREB repressor. Mol Cell Biol 12(12):5394-405	SGD
positive regulation of transcription from RNA polymerase II promoter in response to osmotic stress	IGI: Inferred from Genetic Interaction with SGD:HOG1 Assigned on 2011-12-16 IMP: Inferred from Mutant Phenotype Assigned on 2011-12-16	Pascual-Ahuir A, et al.  (2001) The Sko1p repressor and Gcn4p activator antagonistically modulate stress-regulated transcription in Saccharomyces cerevisiae. Mol Cell Biol 21(1):16-25	SGD
	IGI: Inferred from Genetic Interaction with SGD:HOG1 Assigned on 2011-12-16 IMP: Inferred from Mutant Phenotype Assigned on 2011-12-16	Proft M, et al.  (2001) Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase in response to osmotic stress. EMBO J 20(5):1123-33	SGD
	IGI: Inferred from Genetic Interaction with SGD:HOG1 Assigned on 2011-12-16 IMP: Inferred from Mutant Phenotype Assigned on 2011-12-16	Proft M and Serrano R  (1999) Repressors and upstream repressing sequences of the stress-regulated ENA1 gene in Saccharomyces cerevisiae: bZIP protein Sko1p confers HOG-dependent osmotic regulation. Mol Cell Biol 19(1):537-46	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-16	Garcia-Gimeno MA and Struhl K  (2000) Aca1 and Aca2, ATF/CREB activators in Saccharomyces cerevisiae, are important for carbon source utilization but not the response to stress. Mol Cell Biol 20(12):4340-9	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-12-16 IDA: Inferred from Direct Assay Assigned on 2011-12-16	Proft M and Struhl K  (2002) Hog1 kinase converts the Sko1-Cyc8-Tup1 repressor complex into an activator that recruits SAGA and SWI/SNF in response to osmotic stress. Mol Cell 9(6):1307-17	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytosol	IDA: Inferred from Direct Assay Assigned on 2003-01-27	Pascual-Ahuir A, et al.  (2001) Multiple levels of control regulate the yeast cAMP-response element-binding protein repressor Sko1p in response to stress. J Biol Chem 276(40):37373-8	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2003-01-27	Pascual-Ahuir A, et al.  (2001) Multiple levels of control regulate the yeast cAMP-response element-binding protein repressor Sko1p in response to stress. J Biol Chem 276(40):37373-8	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for SKO1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA binding	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
sequence-specific DNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR004827 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
sequence-specific DNA binding transcription factor activity	IEA: Inferred from Electronic Annotation with EBI:IPR004827 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
regulation of transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:IPR004827 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0805 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transcription, DNA-dependent	IEA: Inferred from Electronic Annotation with EBI:KW-0804 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).