This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-03-20    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
phospholipid binding	ISS: Inferred from Sequence or structural Similarity with MGI:MGI:99665 Assigned on 2008-03-20	Sanchez-Diaz A, et al.  (2008) Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast. Nat Cell Biol 10(4):395-406	SGD
NOT phospholipid binding	IDA: Inferred from Direct Assay Assigned on 2011-05-03	Nishihama R, et al.  (2009) Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae. J Cell Biol 185(6):995-1012	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
barrier septum assembly	IGI: Inferred from Genetic Interaction with SGD:CYK3 Assigned on 2011-05-03 IMP: Inferred from Mutant Phenotype Assigned on 2011-05-03	Nishihama R, et al.  (2009) Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae. J Cell Biol 185(6):995-1012	SGD
chromosome organization	IMP: Inferred from Mutant Phenotype Assigned on 2008-06-20	Ben-Aroya S, et al.  (2008) Toward a comprehensive temperature-sensitive mutant repository of the essential genes of Saccharomyces cerevisiae. Mol Cell 30(2):248-58	SGD
cytokinesis	IPI: Inferred from Physical Interaction with SGD:SHO1, SGD:HOF1, SGD:CYK3 Assigned on 2012-06-20	Labedzka K, et al.  (2012) Sho1p connects the plasma membrane with proteins of the cytokinesis network through multiple isomeric interaction states. J Cell Sci 125(Pt 17):4103-13	SGD
	IGI: Inferred from Genetic Interaction with SGD:CHS2, SGD:CYK3 Assigned on 2013-02-12 IMP: Inferred from Mutant Phenotype Assigned on 2013-02-12	Devrekanli A, et al.  (2012) Inn1 and Cyk3 regulate chitin synthase during cytokinesis in budding yeasts. J Cell Sci 125(Pt 22):5453-66	SGD
mitotic cytokinesis	IMP: Inferred from Mutant Phenotype Assigned on 2008-03-20	Sanchez-Diaz A, et al.  (2008) Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast. Nat Cell Biol 10(4):395-406	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular bud neck contractile ring	IDA: Inferred from Direct Assay Assigned on 2008-03-20	Sanchez-Diaz A, et al.  (2008) Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast. Nat Cell Biol 10(4):395-406	SGD
HICS complex	IPI: Inferred from Physical Interaction with SGD:SHO1, SGD:HOF1, SGD:CYK3 Assigned on 2013-02-14	Labedzka K, et al.  (2012) Sho1p connects the plasma membrane with proteins of the cytokinesis network through multiple isomeric interaction states. J Cell Sci 125(Pt 17):4103-13	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Molecular Function | Cellular Component

High-throughput Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
phospholipid binding	ISS: Inferred from Sequence or structural Similarity Assigned on 2004-01-05	Hazbun TR, et al.  (2003) Assigning function to yeast proteins by integration of technologies. Mol Cell 12(6):1353-65	SGD

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2002-05-07	Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19	SGD
	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cell division	IEA: Inferred from Electronic Annotation with EBI:KW-0132 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitosis	IEA: Inferred from Electronic Annotation with EBI:KW-0498 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular bud neck	IEA: Inferred from Electronic Annotation with EBI:SL-0029 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).