This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-06-09    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity	IDA: Inferred from Direct Assay Assigned on 2008-06-09	Guo S, et al.  (1999) SAC1-like domains of yeast SAC1, INP52, and INP53 and of human synaptojanin encode polyphosphoinositide phosphatases. J Biol Chem 274(19):12990-5	SGD
phosphatidylinositol-3-phosphatase activity	IDA: Inferred from Direct Assay Assigned on 2008-06-09	Guo S, et al.  (1999) SAC1-like domains of yeast SAC1, INP52, and INP53 and of human synaptojanin encode polyphosphoinositide phosphatases. J Biol Chem 274(19):12990-5	SGD
phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity	IMP: Inferred from Mutant Phenotype Assigned on 2008-06-09	Srinivasan S, et al.  (1997) Disruption of three phosphatidylinositol-polyphosphate 5-phosphatase genes from Saccharomyces cerevisiae results in pleiotropic abnormalities of vacuole morphology, cell shape, and osmohomeostasis. Eur J Cell Biol 74(4):350-60	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-02	Guo S, et al.  (1999) SAC1-like domains of yeast SAC1, INP52, and INP53 and of human synaptojanin encode polyphosphoinositide phosphatases. J Biol Chem 274(19):12990-5	SGD
phosphatidylinositol-4-phosphate phosphatase activity	IDA: Inferred from Direct Assay Assigned on 2008-06-09	Guo S, et al.  (1999) SAC1-like domains of yeast SAC1, INP52, and INP53 and of human synaptojanin encode polyphosphoinositide phosphatases. J Biol Chem 274(19):12990-5	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
phosphatidylinositol dephosphorylation	IMP: Inferred from Mutant Phenotype Assigned on 2008-06-09	Srinivasan S, et al.  (1997) Disruption of three phosphatidylinositol-polyphosphate 5-phosphatase genes from Saccharomyces cerevisiae results in pleiotropic abnormalities of vacuole morphology, cell shape, and osmohomeostasis. Eur J Cell Biol 74(4):350-60	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-06-09	Guo S, et al.  (1999) SAC1-like domains of yeast SAC1, INP52, and INP53 and of human synaptojanin encode polyphosphoinositide phosphatases. J Biol Chem 274(19):12990-5	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
actin cortical patch	IDA: Inferred from Direct Assay Assigned on 2003-04-24	Ooms LM, et al.  (2000) The yeast inositol polyphosphate 5-phosphatases inp52p and inp53p translocate to actin patches following hyperosmotic stress: mechanism for regulating phosphatidylinositol 4,5-bisphosphate at plasma membrane invaginations. Mol Cell Biol 20(24):9376-90	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-06-09 IPI: Inferred from Physical Interaction with SGD:ABP1 Assigned on 2008-06-09	Stefan CJ, et al.  (2005) The phosphoinositide phosphatase Sjl2 is recruited to cortical actin patches in the control of vesicle formation and fission during endocytosis. Mol Cell Biol 25(8):2910-23	SGD
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2008-06-09	Ooms LM, et al.  (2000) The yeast inositol polyphosphate 5-phosphatases inp52p and inp53p translocate to actin patches following hyperosmotic stress: mechanism for regulating phosphatidylinositol 4,5-bisphosphate at plasma membrane invaginations. Mol Cell Biol 20(24):9376-90	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mating projection tip	IDA: Inferred from Direct Assay Assigned on 2008-12-19	Narayanaswamy R, et al.  (2009) Systematic Definition of Protein Constituents along the Major Polarization Axis Reveals an Adaptive Reuse of the Polarization Machinery in Pheromone-Treated Budding Yeast. J Proteome Res 8(1):6-19	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0378 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity	IEA: Inferred from Electronic Annotation with IUBMB:3.1.3.36 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
phosphoric ester hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:IPR002013 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
endocytosis	IEA: Inferred from Electronic Annotation with EBI:KW-0254 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
lipid metabolic process	IEA: Inferred from Electronic Annotation with EBI:KW-0443 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
phosphatidylinositol phosphorylation	IEA: Inferred from Electronic Annotation with EBI:IPR000300 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
protein transport	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
actin cortical patch	IEA: Inferred from Electronic Annotation with EBI:SL-0008 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cytoskeleton	IEA: Inferred from Electronic Annotation with EBI:KW-0206 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).