This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2006-09-13    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA-directed DNA polymerase activity	IDA: Inferred from Direct Assay Assigned on 2006-09-13	Johnson LM, et al.  (1985) Isolation of the gene encoding yeast DNA polymerase I. Cell 43(1):369-77	SGD
	IDA: Inferred from Direct Assay Assigned on 2006-09-13	Plevani P, et al.  (1985) Polypeptide structure of DNA primase from a yeast DNA polymerase-primase complex. J Biol Chem 260(11):7102-7	SGD
	IDA: Inferred from Direct Assay Assigned on 2006-09-13	Brooke RG, et al.  (1991) Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activities. J Biol Chem 266(5):3005-15	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA replication	IMP: Inferred from Mutant Phenotype Assigned on 2006-09-13	Budd ME, et al.  (1989) DNA polymerase I is required for premeiotic DNA replication and sporulation but not for X-ray repair in Saccharomyces cerevisiae. Mol Cell Biol 9(2):365-76	SGD
DNA replication initiation	IC: Inferred By Curator from alpha DNA polymerase:primase complex Assigned on 2006-09-13	Plevani P, et al.  (1985) Polypeptide structure of DNA primase from a yeast DNA polymerase-primase complex. J Biol Chem 260(11):7102-7	SGD
DNA synthesis involved in DNA repair	IMP: Inferred from Mutant Phenotype Assigned on 2006-09-13	Budd ME, et al.  (1989) DNA polymerase I is required for premeiotic DNA replication and sporulation but not for X-ray repair in Saccharomyces cerevisiae. Mol Cell Biol 9(2):365-76	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2002-11-01	Holmes AM and Haber JE  (1999) Double-strand break repair in yeast requires both leading and lagging strand DNA polymerases. Cell 96(3):415-24	SGD
lagging strand elongation	IC: Inferred By Curator from alpha DNA polymerase:primase complex Assigned on 2006-09-13	Plevani P, et al.  (1985) Polypeptide structure of DNA primase from a yeast DNA polymerase-primase complex. J Biol Chem 260(11):7102-7	SGD
premeiotic DNA replication	IMP: Inferred from Mutant Phenotype Assigned on 2006-09-13	Budd ME, et al.  (1989) DNA polymerase I is required for premeiotic DNA replication and sporulation but not for X-ray repair in Saccharomyces cerevisiae. Mol Cell Biol 9(2):365-76	SGD
RNA-dependent DNA replication	IDA: Inferred from Direct Assay Assigned on 2007-04-19	Storici F, et al.  (2007) RNA-templated DNA repair. Nature 447(7142):338-41	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
alpha DNA polymerase:primase complex	IDA: Inferred from Direct Assay Assigned on 2006-09-13	Plevani P, et al.  (1985) Polypeptide structure of DNA primase from a yeast DNA polymerase-primase complex. J Biol Chem 260(11):7102-7	SGD
replication fork	IDA: Inferred from Direct Assay Assigned on 2005-07-08	Hiraga S, et al.  (2005) DNA polymerases alpha, delta, and epsilon localize and function together at replication forks in Saccharomyces cerevisiae. Genes Cells 10(4):297-309	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
4 iron, 4 sulfur cluster binding	IEA: Inferred from Electronic Annotation with EBI:KW-0004 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR004578, EBI:IPR006134 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DNA-directed DNA polymerase activity	IEA: Inferred from Electronic Annotation with EBI:IPR015088, EBI:IPR006133, EBI:IPR017964, EBI:IPR004578, EBI:IPR006172, EBI:IPR006134 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0239 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with IUBMB:2.7.7.7 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
iron-sulfur cluster binding	IEA: Inferred from Electronic Annotation with EBI:KW-0411 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleic acid binding	IEA: Inferred from Electronic Annotation with EBI:IPR012337, EBI:IPR017964, EBI:IPR006172 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleoside binding	IEA: Inferred from Electronic Annotation with EBI:IPR015088 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:IPR017964, EBI:IPR004578, EBI:IPR006172, EBI:IPR006134 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleotidyltransferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0548 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA replication	IEA: Inferred from Electronic Annotation with EBI:IPR015088, EBI:IPR006133, EBI:IPR004578, EBI:IPR006134 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0235 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DNA replication checkpoint	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10322_AN3 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
DNA replication, synthesis of RNA primer	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10322_AN3 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
DNA strand elongation involved in DNA replication	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10322_AN3 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
gene conversion at mating-type locus, DNA repair synthesis	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10322_AN45 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
nucleobase-containing compound metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR017964, EBI:IPR006172 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
S phase of mitotic cell cycle	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10322_AN3 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).