TOP2/YNL088W Gene Ontology Annotations

This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

TOP2 Manually curated*:

Jump to: top | High-throughput | Computational

Last Reviewed on: 2003-08-14 Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA topoisomerase type II (ATP-hydrolyzing) activity	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Goto T, et al. (1984) The purification and characterization of DNA topoisomerases I and II of the yeast Saccharomyces cerevisiae. J Biol Chem 259(16):10422-9	SGD
DNA topoisomerase type II (ATP-hydrolyzing) activity	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Goto T and Wang JC (1984) Yeast DNA topoisomerase II is encoded by a single-copy, essential gene. Cell 36(4):1073-80	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin assembly or disassembly	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Garinther WI and Schultz MC (1997) Topoisomerase function during replication-independent chromatin assembly in yeast. Mol Cell Biol 17(7):3520-6	SGD
chromatin remodeling at centromere	IMP: Inferred from Mutant Phenotype Assigned on 2008-08-27	Warsi TH, et al. (2008) DNA topoisomerase II is a determinant of the tensile properties of yeast centromeric chromatin and the tension checkpoint. Mol Biol Cell 19(10):4421-33	SGD
DNA strand elongation involved in DNA replication	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Kim RA and Wang JC (1989) Function of DNA topoisomerases as replication swivels in Saccharomyces cerevisiae. J Mol Biol 208(2):257-67	SGD
DNA topological change	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Goto T, et al. (1984) The purification and characterization of DNA topoisomerases I and II of the yeast Saccharomyces cerevisiae. J Biol Chem 259(16):10422-9	SGD
mitotic DNA integrity checkpoint	IMP: Inferred from Mutant Phenotype Assigned on 2010-07-29	Andrews CA, et al. (2006) A mitotic topoisomerase II checkpoint in budding yeast is required for genome stability but acts independently of Pds1/securin. Genes Dev 20(9):1162-74	SGD
reciprocal meiotic recombination	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	Rose D, et al. (1990) Segregation of recombined chromosomes in meiosis I requires DNA topoisomerase II. Cell 60(6):1009-17	SGD
regulation of mitotic recombination	IMP: Inferred from Mutant Phenotype Assigned on 2003-08-14	Christman MF, et al. (1988) Mitotic recombination in the rDNA of S. cerevisiae is suppressed by the combined action of DNA topoisomerases I and II. Cell 55(3):413-25	SGD
replication fork progression beyond termination site	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-05	Fachinetti D, et al. (2010) Replication Termination at Eukaryotic Chromosomes Is Mediated by Top2 and Occurs at Genomic Loci Containing Pausing Elements. Mol Cell 39(4):595-605	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA replication termination region	IPI: Inferred from Physical Interaction Assigned on 2011-05-05	Fachinetti D, et al. (2010) Replication Termination at Eukaryotic Chromosomes Is Mediated by Top2 and Occurs at Genomic Loci Containing Pausing Elements. Mol Cell 39(4):595-605	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Goto T, et al. (1984) The purification and characterization of DNA topoisomerases I and II of the yeast Saccharomyces cerevisiae. J Biol Chem 259(16):10422-9	SGD
synaptonemal complex	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Klein F, et al. (1992) Localization of RAP1 and topoisomerase II in nuclei and meiotic chromosomes of yeast. J Cell Biol 117(5):935-48	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

TOP2 High-throughput**:

Jump to: top | Manually curated | Computational

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2012-12-12	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

TOP2 Computational***:

Jump to: top | Manually curated | High-throughput

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:IPR018522, EBI:IPR002205, EBI:IPR013758, EBI:IPR013757, EBI:IPR013506, EBI:IPR003594, EBI:IPR013759, EBI:IPR001241, EBI:IPR001154, EBI:IPR013760 Last updated 2013-03-02	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
ATP binding	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR018522, EBI:IPR002205, EBI:IPR013758, EBI:IPR013757, EBI:IPR013506, EBI:IPR013759, EBI:IPR001241, EBI:IPR001154, EBI:IPR013760 Last updated 2013-03-02	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
DNA binding	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DNA topoisomerase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0799 Last updated 2013-03-02	UniProt-GOA (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DNA topoisomerase type II (ATP-hydrolyzing) activity	IEA: Inferred from Electronic Annotation with EBI:IPR018522, EBI:IPR002205, EBI:IPR013758, EBI:IPR013757, EBI:IPR013506, EBI:IPR013759, EBI:IPR001241, EBI:IPR001154, EBI:IPR013760 Last updated 2013-03-02	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
DNA topoisomerase type II (ATP-hydrolyzing) activity	IEA: Inferred from Electronic Annotation with IUBMB:5.99.1.3 Last updated 2013-03-02	GOA curators and MGI curators (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
DNA-dependent ATPase activity	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10169_AN0 Last updated 2011-10-06	Gaudet P, et al. (2011) Annotation inferences using phylogenetic trees.	RefGenome
isomerase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0413 Last updated 2013-03-02	UniProt-GOA (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA metabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR013758, EBI:IPR013757 Last updated 2013-03-02	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
DNA topological change	IEA: Inferred from Electronic Annotation with EBI:IPR018522, EBI:IPR002205, EBI:IPR013758, EBI:IPR013757, EBI:IPR013506, EBI:IPR013759, EBI:IPR001241, EBI:IPR001154, EBI:IPR013760 Last updated 2013-03-02	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
resolution of meiotic recombination intermediates	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10169_AN42 Last updated 2011-10-06	Gaudet P, et al. (2011) Annotation inferences using phylogenetic trees.	RefGenome
sister chromatid segregation	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10169_AN42 Last updated 2011-10-06	Gaudet P, et al. (2011) Annotation inferences using phylogenetic trees.	RefGenome

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
chromosome	IEA: Inferred from Electronic Annotation with EBI:IPR002205, EBI:IPR013758, EBI:IPR013757, EBI:IPR013506, EBI:IPR013759, EBI:IPR001241, EBI:IPR001154, EBI:IPR013760 Last updated 2013-03-02	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
DNA topoisomerase complex (ATP-hydrolyzing)	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10169_AN0 Last updated 2011-10-06	Gaudet P, et al. (2011) Annotation inferences using phylogenetic trees.	RefGenome
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).