This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2010-03-04    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
protein tyrosine/serine/threonine phosphatase activity	IDA: Inferred from Direct Assay Assigned on 2010-02-27	Doi K, et al.  (1994) MSG5, a novel protein phosphatase promotes adaptation to pheromone response in S. cerevisiae. EMBO J 13(1):61-70	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion	IMP: Inferred from Mutant Phenotype Assigned on 2007-06-01	Blackwell E, et al.  (2003) Effect of the pheromone-responsive G(alpha) and phosphatase proteins of Saccharomyces cerevisiae on the subcellular localization of the Fus3 mitogen-activated protein kinase. Mol Cell Biol 23(4):1135-50	SGD
	IGI: Inferred from Genetic Interaction with SGD:GPA1 Assigned on 2010-02-27 IMP: Inferred from Mutant Phenotype Assigned on 2002-12-06	Doi K, et al.  (1994) MSG5, a novel protein phosphatase promotes adaptation to pheromone response in S. cerevisiae. EMBO J 13(1):61-70	SGD
inactivation of MAPK activity involved in cell wall biogenesis	IGI: Inferred from Genetic Interaction with SGD:MKK1 Assigned on 2013-04-29	Hahn JS and Thiele DJ  (2002) Regulation of the Saccharomyces cerevisiae Slt2 kinase pathway by the stress-inducible Sdp1 dual specificity phosphatase. J Biol Chem 277(24):21278-84	SGD
	IGI: Inferred from Genetic Interaction with SGD:SAC7 Assigned on 2010-02-27 IMP: Inferred from Mutant Phenotype Assigned on 2010-02-27	Martin H, et al.  (2000) Regulatory mechanisms for modulation of signaling through the cell integrity Slt2-mediated pathway in Saccharomyces cerevisiae. J Biol Chem 275(2):1511-9	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-02-27 IDA: Inferred from Direct Assay Assigned on 2010-02-27 IPI: Inferred from Physical Interaction with SGD:SLT2 Assigned on 2010-02-27	Flandez M, et al.  (2004) Reciprocal regulation between Slt2 MAPK and isoforms of Msg5 dual-specificity protein phosphatase modulates the yeast cell integrity pathway. J Biol Chem 279(12):11027-34	SGD
inactivation of MAPK activity involved in conjugation with cellular fusion	IMP: Inferred from Mutant Phenotype Assigned on 2010-02-27 IDA: Inferred from Direct Assay Assigned on 2010-02-27	Doi K, et al.  (1994) MSG5, a novel protein phosphatase promotes adaptation to pheromone response in S. cerevisiae. EMBO J 13(1):61-70	SGD
protein dephosphorylation	IMP: Inferred from Mutant Phenotype Assigned on 2010-02-27 IDA: Inferred from Direct Assay Assigned on 2010-02-27	Doi K, et al.  (1994) MSG5, a novel protein phosphatase promotes adaptation to pheromone response in S. cerevisiae. EMBO J 13(1):61-70	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-02-27 IDA: Inferred from Direct Assay Assigned on 2004-06-17	Flandez M, et al.  (2004) Reciprocal regulation between Slt2 MAPK and isoforms of Msg5 dual-specificity protein phosphatase modulates the yeast cell integrity pathway. J Biol Chem 279(12):11027-34	SGD
regulation of fungal-type cell wall organization	IMP: Inferred from Mutant Phenotype Assigned on 2010-02-27	Flandez M, et al.  (2004) Reciprocal regulation between Slt2 MAPK and isoforms of Msg5 dual-specificity protein phosphatase modulates the yeast cell integrity pathway. J Biol Chem 279(12):11027-34	SGD
regulation of MAPK export from nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2010-03-04	Blackwell E, et al.  (2003) Effect of the pheromone-responsive G(alpha) and phosphatase proteins of Saccharomyces cerevisiae on the subcellular localization of the Fus3 mitogen-activated protein kinase. Mol Cell Biol 23(4):1135-50	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-03-04	Blackwell E, et al.  (2007) The pheromone-induced nuclear accumulation of the Fus3 MAPK in yeast depends on its phosphorylation state and on Dig1 and Dig2. BMC Cell Biol 8:44	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2007-12-17	Maeder CI, et al.  (2007) Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling. Nat Cell Biol 9(11):1319-1326	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-11-30	Blackwell E, et al.  (2007) The pheromone-induced nuclear accumulation of the Fus3 MAPK in yeast depends on its phosphorylation state and on Dig1 and Dig2. BMC Cell Biol 8:44	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2007-12-17	Maeder CI, et al.  (2007) Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling. Nat Cell Biol 9(11):1319-1326	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-11-30	Blackwell E, et al.  (2007) The pheromone-induced nuclear accumulation of the Fus3 MAPK in yeast depends on its phosphorylation state and on Dig1 and Dig2. BMC Cell Biol 8:44	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0378 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
MAP kinase tyrosine/serine/threonine phosphatase activity	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10159_AN957 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
phosphatase activity	IEA: Inferred from Electronic Annotation with EBI:IPR016130, EBI:IPR000387 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
phosphoprotein phosphatase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0904 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein tyrosine phosphatase activity	IEA: Inferred from Electronic Annotation with EBI:IPR016130 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:3.1.3.48 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
protein tyrosine/serine/threonine phosphatase activity	IEA: Inferred from Electronic Annotation with EBI:IPR024950, EBI:IPR000340, EBI:IPR020422 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
dephosphorylation	IEA: Inferred from Electronic Annotation with EBI:IPR016130, EBI:IPR000387 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
peptidyl-tyrosine dephosphorylation	IEA: Inferred from Electronic Annotation with EBI:IPR016130 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	GOC
	IEA: Inferred from Electronic Annotation with IUBMB:3.1.3.48 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	GOC
protein dephosphorylation	IEA: Inferred from Electronic Annotation with EBI:IPR024950, EBI:IPR000340, EBI:IPR020422 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10159_AN957 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytosol	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10159_AN957 Last updated 2011-10-06	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).