This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-02-13    Molecular Function | Biological Process

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
phosphopyruvate hydratase activity	IGI: Inferred from Genetic Interaction with SGD:ENO2, SGD:ENO1 Assigned on 2013-02-15 IDA: Inferred from Direct Assay Assigned on 2013-02-15	Kornblatt MJ, et al.  (2013) The Saccharomyces cerevisiae enolase-related regions encode proteins that are active enolases. Yeast 30(2):55-69	SGD
	ISS: Inferred from Sequence or structural Similarity Assigned on 2003-02-13	Tatusov RL, et al.  (2000) The COG database: a tool for genome-scale analysis of protein functions and evolution. Nucleic Acids Res 28(1):33-6	SGD
	ISA: Inferred from Sequence Alignment with SGD:ENO2, SGD:ENO1 Assigned on 2010-03-19	Pryde FE, et al.  (1995) Sequence analysis of the right end of chromosome XV in Saccharomyces cerevisiae: an insight into the structural and functional significance of sub-telomeric repeat sequences. Yeast 11(4):371-82	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
biological_process unknown	ND: No Biological Data Available Assigned on 2002-10-02	SGD  (2002) Use of the ND evidence code for Gene Ontology (GO) terms in SGD ()	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2002-05-07	Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
lyase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0456 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
magnesium ion binding	IEA: Inferred from Electronic Annotation with EBI:IPR000941, EBI:IPR020810, EBI:IPR020809, EBI:IPR020811 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
phosphopyruvate hydratase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000941, EBI:IPR020810, EBI:IPR020809, EBI:IPR020811 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:4.2.1.11 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
glycolysis	IEA: Inferred from Electronic Annotation with EBI:IPR000941, EBI:IPR020810, EBI:IPR020809, EBI:IPR020811 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with UniPathway:UPA00109 Last updated 2013-03-02	UniProt-GOA  (2012) Gene Ontology annotation based on UniPathway vocabulary mapping.	UniPathway
	IEA: Inferred from Electronic Annotation with EBI:KW-0324 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
phosphopyruvate hydratase complex	IEA: Inferred from Electronic Annotation with EBI:IPR000941, EBI:IPR020810, EBI:IPR020809, EBI:IPR020811 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).