This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2002-06-24    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
nuclear localization sequence binding	IDA: Inferred from Direct Assay Assigned on 2012-02-22	Mosammaparast N, et al.  (2002) Pathways mediating the nuclear import of histones H3 and H4 in yeast. J Biol Chem 277(1):862-8	SGD
protein transporter activity	IMP: Inferred from Mutant Phenotype Assigned on 2009-10-20 IPI: Inferred from Physical Interaction with SGD:YAP1 Assigned on 2009-10-20	Isoyama T, et al.  (2001) Nuclear import of the yeast AP-1-like transcription factor Yap1p is mediated by transport receptor Pse1p, and this import step is not affected by oxidative stress. J Biol Chem 276(24):21863-9	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
mRNA export from nucleus	IGI: Inferred from Genetic Interaction with SGD:KAP123 Assigned on 2009-10-20	Seedorf M and Silver PA  (1997) Importin/karyopherin protein family members required for mRNA export from the nucleus. Proc Natl Acad Sci U S A 94(16):8590-5	SGD
protein import into nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2006-05-22	Lebreton A, et al.  (2006) A functional network involved in the recycling of nucleocytoplasmic pre-60S factors. J Cell Biol 173(3):349-60	SGD
regulation of mitosis	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-25	Makhnevych T, et al.  (2003) Cell cycle regulated transport controlled by alterations in the nuclear pore complex. Cell 115(7):813-23	SGD
regulation of protein desumoylation	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-25 IPI: Inferred from Physical Interaction with SGD:ULP1 Assigned on 2011-05-25	Makhnevych T, et al.  (2007) The role of karyopherins in the regulated sumoylation of septins. J Cell Biol 177(1):39-49	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2002-06-24	Seedorf M and Silver PA  (1997) Importin/karyopherin protein family members required for mRNA export from the nucleus. Proc Natl Acad Sci U S A 94(16):8590-5	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2002-06-24	Seedorf M and Silver PA  (1997) Importin/karyopherin protein family members required for mRNA export from the nucleus. Proc Natl Acad Sci U S A 94(16):8590-5	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	ISM: Inferred from Sequence Model Assigned on 2011-05-31	De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein transport	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).