This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-08-12    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity	IDA: Inferred from Direct Assay Assigned on 2009-05-20	Frechin M, et al.  (2009) Yeast mitochondrial Gln-tRNA(Gln) is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS. Genes Dev 23(9):1119-30	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
endoplasmic reticulum organization	IGI: Inferred from Genetic Interaction with SGD:HMG2 Assigned on 2008-08-12	Federovitch CM, et al.  (2008) Genetic and structural analysis of Hmg2p-induced endoplasmic reticulum remodeling in Saccharomyces cerevisiae. Mol Biol Cell 19(10):4506-20	SGD
glutaminyl-tRNAGln biosynthesis via transamidation	IDA: Inferred from Direct Assay Assigned on 2009-06-25	Frechin M, et al.  (2009) Yeast mitochondrial Gln-tRNA(Gln) is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS. Genes Dev 23(9):1119-30	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
glutamyl-tRNA(Gln) amidotransferase complex	IDA: Inferred from Direct Assay Assigned on 2009-05-20	Frechin M, et al.  (2009) Yeast mitochondrial Gln-tRNA(Gln) is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS. Genes Dev 23(9):1119-30	SGD
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2009-05-20	Frechin M, et al.  (2009) Yeast mitochondrial Gln-tRNA(Gln) is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS. Genes Dev 23(9):1119-30	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2002-08-06	Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19	SGD
	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD
	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-09-24	Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ATP binding	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
carbon-nitrogen ligase activity, with glutamine as amido-N-donor	IEA: Inferred from Electronic Annotation with EBI:IPR000120 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with HAMAP:MF_03150 Last updated 2013-03-02	Swiss Institute of Bioinformatics (SIB) curators and GOA curators  (2006) Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins	UniProtKB
glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity	IEA: Inferred from Electronic Annotation with HAMAP:MF_03150 Last updated 2013-03-02	Swiss Institute of Bioinformatics (SIB) curators and GOA curators  (2006) Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins	UniProtKB
ligase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0436 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
glutaminyl-tRNAGln biosynthesis via transamidation	IEA: Inferred from Electronic Annotation with HAMAP:MF_03150 Last updated 2013-03-02	Swiss Institute of Bioinformatics (SIB) curators and GOA curators  (2006) Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins	UniProtKB
mitochondrial translation	IEA: Inferred from Electronic Annotation with HAMAP:MF_03150 Last updated 2013-03-02	Swiss Institute of Bioinformatics (SIB) curators and GOA curators  (2006) Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins	UniProtKB
translation	IEA: Inferred from Electronic Annotation with EBI:IPR004412 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0648 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
glutamyl-tRNA(Gln) amidotransferase complex	IEA: Inferred from Electronic Annotation with HAMAP:MF_03150 Last updated 2013-03-02	Swiss Institute of Bioinformatics (SIB) curators and GOA curators  (2006) Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins	UniProtKB
mitochondrion	IEA: Inferred from Electronic Annotation with EBI:SL-0173 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with HAMAP:MF_03150 Last updated 2013-03-02	Swiss Institute of Bioinformatics (SIB) curators and GOA curators  (2006) Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0496 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).