This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2004-01-28    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
microtubule motor activity	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-02-08 IMP: Inferred from Mutant Phenotype Assigned on 2002-02-08	Manning BD, et al.  (1999) Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p. J Cell Biol 144(6):1219-33	SGD
contributes_to minus-end-directed microtubule motor activity	IDA: Inferred from Direct Assay Assigned on 2010-09-15	Chu HM, et al.  (2005) Kar3 interaction with Cik1 alters motor structure and function. EMBO J 24(18):3214-23	SGD
protein heterodimerization activity	IPI: Inferred from Physical Interaction with SGD:KAR3 Assigned on 2010-09-15	Chu HM, et al.  (2005) Kar3 interaction with Cik1 alters motor structure and function. EMBO J 24(18):3214-23	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
establishment of mitotic spindle orientation	TAS: Traceable Author Statement Assigned on 2004-01-28	Cottingham FR, et al.  (1999) Novel roles for saccharomyces cerevisiae mitotic spindle motors. J Cell Biol 147(2):335-50	SGD
karyogamy involved in conjugation with cellular fusion	IMP: Inferred from Mutant Phenotype Assigned on 2006-10-18	Page BD and Snyder M  (1992) CIK1: a developmentally regulated spindle pole body-associated protein important for microtubule functions in Saccharomyces cerevisiae. Genes Dev 6(8):1414-29	SGD
meiosis	IMP: Inferred from Mutant Phenotype Assigned on 2002-02-08 IDA: Inferred from Direct Assay Assigned on 2002-02-08	Shanks RM, et al.  (2001) The Kar3-interacting protein Cik1p plays a critical role in passage through meiosis I in Saccharomyces cerevisiae. Genetics 159(3):939-51	SGD
mitotic sister chromatid segregation	TAS: Traceable Author Statement Assigned on 2001-01-18	Manning BD, et al.  (1999) Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p. J Cell Biol 144(6):1219-33	SGD
mitotic spindle organization in nucleus	TAS: Traceable Author Statement Assigned on 2001-01-18	Manning BD, et al.  (1999) Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p. J Cell Biol 144(6):1219-33	SGD
nuclear migration involved in conjugation with cellular fusion	IMP: Inferred from Mutant Phenotype Assigned on 2009-05-04	Zaichick SV, et al.  (2009) The mating-specific Galpha interacts with a kinesin-14 and regulates pheromone-induced nuclear migration in budding yeast. Mol Biol Cell 20(12):2820-30	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasmic microtubule	TAS: Traceable Author Statement Assigned on 2001-01-18	Winsor B and Schiebel E  (1997) Review: an overview of the Saccharomyces cerevisiae microtubule and microfilament cytoskeleton. Yeast 13(5):399-434	SGD
kinesin complex	IDA: Inferred from Direct Assay Assigned on 2002-02-08	Manning BD, et al.  (1999) Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p. J Cell Biol 144(6):1219-33	SGD
spindle	IDA: Inferred from Direct Assay Assigned on 2002-02-08	Shanks RM, et al.  (2001) The Kar3-interacting protein Cik1p plays a critical role in passage through meiosis I in Saccharomyces cerevisiae. Genetics 159(3):939-51	SGD
spindle pole body	IDA: Inferred from Direct Assay Assigned on 2002-02-08	Page BD and Snyder M  (1992) CIK1: a developmentally regulated spindle pole body-associated protein important for microtubule functions in Saccharomyces cerevisiae. Genes Dev 6(8):1414-29	SGD
	IDA: Inferred from Direct Assay Assigned on 2002-02-08	Shanks RM, et al.  (2001) The Kar3-interacting protein Cik1p plays a critical role in passage through meiosis I in Saccharomyces cerevisiae. Genetics 159(3):939-51	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for CIK1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Cellular Component

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cytoskeleton	IEA: Inferred from Electronic Annotation with EBI:KW-0206 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
microtubule	IEA: Inferred from Electronic Annotation with EBI:KW-0493 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
spindle pole body	IEA: Inferred from Electronic Annotation with EBI:SL-0252 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).