This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-05-30    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
contributes_to Atg8 ligase activity	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-30	Matsushita M, et al.  (2007) Structure of Atg5{middle dot}Atg16, a Complex Essential for Autophagy. J Biol Chem 282(9):6763-72	SGD
protein binding, bridging	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-30	Kuma A, et al.  (2002) Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast. J Biol Chem 277(21):18619-25	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
C-terminal protein lipidation	IMP: Inferred from Mutant Phenotype Assigned on 2009-12-09	Matsushita M, et al.  (2007) Structure of Atg5{middle dot}Atg16, a Complex Essential for Autophagy. J Biol Chem 282(9):6763-72	SGD
CVT pathway	IMP: Inferred from Mutant Phenotype Assigned on 2010-01-14	Matsushita M, et al.  (2007) Structure of Atg5{middle dot}Atg16, a Complex Essential for Autophagy. J Biol Chem 282(9):6763-72	SGD
macroautophagy	IDA: Inferred from Direct Assay Assigned on 2008-05-30	Kuma A, et al.  (2002) Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast. J Biol Chem 277(21):18619-25	SGD
mitochondrion degradation	IMP: Inferred from Mutant Phenotype Assigned on 2010-01-07	Kanki T, et al.  (2009) A genomic screen for yeast mutants defective in selective mitochondria autophagy. Mol Biol Cell 20(22):4730-8	SGD
piecemeal microautophagy of nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2008-08-28	Krick R, et al.  (2008) Piecemeal microautophagy of the nucleus requires the core macroautophagy genes. Mol Biol Cell 19(10):4492-505	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
Atg12-Atg5-Atg16 complex	IPI: Inferred from Physical Interaction with SGD:ATG5 Assigned on 2008-05-30	Mizushima N, et al.  (1999) Apg16p is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway. EMBO J 18(14):3888-96	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-05-13	Kuma A, et al.  (2002) Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast. J Biol Chem 277(21):18619-25	SGD
pre-autophagosomal structure	IDA: Inferred from Direct Assay Assigned on 2008-05-30	Suzuki K, et al.  (2001) The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation. EMBO J 20(21):5971-81	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2012-12-12	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2012-12-12	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
autophagy	IEA: Inferred from Electronic Annotation with EBI:KW-0072 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein transport	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasmic membrane-bounded vesicle	IEA: Inferred from Electronic Annotation with EBI:SL-0088 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
cytoplasmic vesicle	IEA: Inferred from Electronic Annotation with EBI:KW-0968 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
vacuole	IEA: Inferred from Electronic Annotation with EBI:SL-0272 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0926 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).