This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2005-11-08    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
G-protein alpha-subunit binding	IPI: Inferred from Physical Interaction with SGD:GPA2 Assigned on 2007-11-30	Zeller CE, et al.  (2007) The RACK1 ortholog Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast. J Biol Chem 282(34):25168-76	SGD
GDP-dissociation inhibitor activity	IDA: Inferred from Direct Assay Assigned on 2007-11-30	Zeller CE, et al.  (2007) The RACK1 ortholog Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast. J Biol Chem 282(34):25168-76	SGD
signal transducer activity	IMP: Inferred from Mutant Phenotype Assigned on 2007-07-09 IPI: Inferred from Physical Interaction with SGD:GPA2 Assigned on 2007-07-09	Zeller CE, et al.  (2007) The RACK1 ortholog Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast. J Biol Chem 282(34):25168-76	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
G-protein coupled receptor signaling pathway	IMP: Inferred from Mutant Phenotype Assigned on 2007-11-30 IPI: Inferred from Physical Interaction with SGD:GPA2 Assigned on 2007-11-30	Zeller CE, et al.  (2007) The RACK1 ortholog Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast. J Biol Chem 282(34):25168-76	SGD
glucose mediated signaling pathway	IMP: Inferred from Mutant Phenotype Assigned on 2007-11-30 IPI: Inferred from Physical Interaction with SGD:CYR1 Assigned on 2007-11-30	Zeller CE, et al.  (2007) The RACK1 ortholog Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast. J Biol Chem 282(34):25168-76	SGD
invasive growth in response to glucose limitation	IMP: Inferred from Mutant Phenotype Assigned on 2007-07-09	Zeller CE, et al.  (2007) The RACK1 ortholog Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast. J Biol Chem 282(34):25168-76	SGD
negative regulation of translation	IMP: Inferred from Mutant Phenotype Assigned on 2005-11-08	Gerbasi VR, et al.  (2004) Yeast Asc1p and mammalian RACK1 are functionally orthologous core 40S ribosomal proteins that repress gene expression. Mol Cell Biol 24(18):8276-87	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytosolic small ribosomal subunit	IDA: Inferred from Direct Assay Assigned on 2005-11-08	Gerbasi VR, et al.  (2004) Yeast Asc1p and mammalian RACK1 are functionally orthologous core 40S ribosomal proteins that repress gene expression. Mol Cell Biol 24(18):8276-87	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2002-05-07	Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Cellular Component

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).