This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2006-03-14    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
GTPase activator activity	TAS: Traceable Author Statement Assigned on 2003-01-31	Jensen S, et al.  (2002) Spatial regulation of the guanine nucleotide exchange factor Lte1 in Saccharomyces cerevisiae. J Cell Sci 115(Pt 24):4977-91	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
mitotic spindle orientation checkpoint	IGI: Inferred from Genetic Interaction Assigned on 2006-03-14 IMP: Inferred from Mutant Phenotype Assigned on 2006-03-14	D'Aquino KE, et al.  (2005) The protein kinase Kin4 inhibits exit from mitosis in response to spindle position defects. Mol Cell 19(2):223-34	SGD
regulation of exit from mitosis	TAS: Traceable Author Statement Assigned on 2006-03-14	D'Aquino KE, et al.  (2005) The protein kinase Kin4 inhibits exit from mitosis in response to spindle position defects. Mol Cell 19(2):223-34	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
spindle pole body	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Fraschini R, et al.  (1999) Budding yeast Bub2 is localized at spindle pole bodies and activates the mitotic checkpoint via a different pathway from Mad2. J Cell Biol 145(5):979-91	SGD
	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Li R  (1999) Bifurcation of the mitotic checkpoint pathway in budding yeast. Proc Natl Acad Sci U S A 96(9):4989-94	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for BUB2

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
Rab GTPase activator activity	IEA: Inferred from Electronic Annotation with EBI:IPR000195 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cell division	IEA: Inferred from Electronic Annotation with EBI:KW-0132 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitosis	IEA: Inferred from Electronic Annotation with EBI:KW-0498 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
positive regulation of Rab GTPase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000195 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	GOC
regulation of Rab GTPase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000195 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
cytoskeleton	IEA: Inferred from Electronic Annotation with EBI:KW-0206 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
intracellular	IEA: Inferred from Electronic Annotation with EBI:IPR000195 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
spindle pole	IEA: Inferred from Electronic Annotation with EBI:SL-0448 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).