This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-10-20    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
7S RNA binding	IDA: Inferred from Direct Assay Assigned on 2008-07-28	Van Nues RW and Brown JD  (2004) Saccharomyces SRP RNA secondary structures: a conserved S-domain and extended Alu-domain. RNA 10(1):75-89	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein targeting to ER	IMP: Inferred from Mutant Phenotype Assigned on 2003-10-20	Stirling CJ and Hewitt EW  (1992) The S. cerevisiae SEC65 gene encodes a component of yeast signal recognition particle with homology to human SRP19. Nature 356(6369):534-7	SGD
SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition	IMP: Inferred from Mutant Phenotype Assigned on 2002-12-02	Hann BC, et al.  (1992) SEC65 gene product is a subunit of the yeast signal recognition particle required for its integrity. Nature 356(6369):532-3	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
signal recognition particle, endoplasmic reticulum targeting	ISS: Inferred from Sequence or structural Similarity Assigned on 2003-10-20 IMP: Inferred from Mutant Phenotype Assigned on 2003-10-20	Stirling CJ and Hewitt EW  (1992) The S. cerevisiae SEC65 gene encodes a component of yeast signal recognition particle with homology to human SRP19. Nature 356(6369):534-7	SGD
	IDA: Inferred from Direct Assay Assigned on 2002-12-02	Hann BC, et al.  (1992) SEC65 gene product is a subunit of the yeast signal recognition particle required for its integrity. Nature 356(6369):532-3	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for SEC65

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
7S RNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR002778 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
SRP-dependent cotranslational protein targeting to membrane	IEA: Inferred from Electronic Annotation with EBI:IPR002778 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
ribonucleoprotein complex	IEA: Inferred from Electronic Annotation with EBI:KW-0687 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
signal recognition particle	IEA: Inferred from Electronic Annotation with EBI:IPR002778 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
signal recognition particle, endoplasmic reticulum targeting	IEA: Inferred from Electronic Annotation with EBI:KW-0733 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).