This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-07-10    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
recombinase activity	IDA: Inferred from Direct Assay Assigned on 2004-10-08	Bi B, et al.  (2004) Human and yeast Rad52 proteins promote DNA strand exchange. Proc Natl Acad Sci U S A 101(26):9568-72	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA recombinase assembly	IDA: Inferred from Direct Assay Assigned on 2002-09-25 TAS: Traceable Author Statement Assigned on 2002-09-25	Gasior SL, et al.  (2001) Assembly of RecA-like recombinases: distinct roles for mediator proteins in mitosis and meiosis. Proc Natl Acad Sci U S A 98(15):8411-8	SGD
DNA strand renaturation	IDA: Inferred from Direct Assay Assigned on 2012-09-12	Shinohara A, et al.  (1998) Rad52 forms ring structures and co-operates with RPA in single-strand DNA annealing. Genes Cells 3(3):145-56	SGD
double-strand break repair via break-induced replication	TAS: Traceable Author Statement Assigned on 2002-09-25	Kraus E, et al.  (2001) Break-induced replication: a review and an example in budding yeast. Proc Natl Acad Sci U S A 98(15):8255-62	SGD
double-strand break repair via single-strand annealing	IGI: Inferred from Genetic Interaction Assigned on 2002-09-25	Davis AP and Symington LS  (2001) The yeast recombinational repair protein Rad59 interacts with Rad52 and stimulates single-strand annealing. Genetics 159(2):515-25	SGD
double-strand break repair via synthesis-dependent strand annealing	TAS: Traceable Author Statement Assigned on 2002-08-14	Paques F and Haber JE  (1999) Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 63(2):349-404	SGD
meiotic DNA recombinase assembly	TAS: Traceable Author Statement Assigned on 2002-07-08	Gasior SL, et al.  (2001) Assembly of RecA-like recombinases: distinct roles for mediator proteins in mitosis and meiosis. Proc Natl Acad Sci U S A 98(15):8411-8	SGD
meiotic joint molecule formation	IGI: Inferred from Genetic Interaction with SGD:NDT80 Assigned on 2008-08-28 IMP: Inferred from Mutant Phenotype Assigned on 2008-08-28	Lao JP, et al.  (2008) Rad52 promotes postinvasion steps of meiotic double-strand-break repair. Mol Cell 29(4):517-24	SGD
postreplication repair	IMP: Inferred from Mutant Phenotype Assigned on 2006-05-08	Prakash L  (1981) Characterization of postreplication repair in Saccharomyces cerevisiae and effects of rad6, rad18, rev3 and rad52 mutations. Mol Gen Genet 184(3):471-8	SGD
telomere maintenance via recombination	IMP: Inferred from Mutant Phenotype Assigned on 2002-09-25	Chen Q, et al.  (2001) Two survivor pathways that allow growth in the absence of telomerase are generated by distinct telomere recombination events. Mol Cell Biol 21(5):1819-27	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nuclear chromosome	IDA: Inferred from Direct Assay Assigned on 2003-07-10	Lisby M, et al.  (2003) Colocalization of multiple DNA double-strand breaks at a single Rad52 repair centre. Nat Cell Biol 5(6):572-7	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2002-09-05	Lisby M, et al.  (2001) Rad52 forms DNA repair and recombination centers during S phase. Proc Natl Acad Sci U S A 98(15):8276-82	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IDA: Inferred from Direct Assay Assigned on 2012-12-12	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA recombination	IEA: Inferred from Electronic Annotation with EBI:IPR007232, EBI:IPR004585 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0233 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DNA repair	IEA: Inferred from Electronic Annotation with EBI:IPR007232, EBI:IPR004585 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0234 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
response to DNA damage stimulus	IEA: Inferred from Electronic Annotation with EBI:KW-0227 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).