This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-04-02    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
peroxiredoxin activity	IDA: Inferred from Direct Assay Assigned on 2007-01-18	Ogusucu R, et al.  (2007) Reactions of yeast thioredoxin peroxidases I and II with hydrogen peroxide and peroxynitrite: rate constants by competitive kinetics. Free Radic Biol Med 42(3):326-34	SGD
ribosome binding	IDA: Inferred from Direct Assay Assigned on 2008-04-02	Trotter EW, et al.  (2008) The yeast Tsa1 peroxiredoxin is a ribosome-associated antioxidant. Biochem J 412(1):73-80	SGD
thioredoxin peroxidase activity	IMP: Inferred from Mutant Phenotype Assigned on 2005-06-22 IDA: Inferred from Direct Assay Assigned on 2005-06-22	Chae HZ, et al.  (1994) Thioredoxin-dependent peroxide reductase from yeast. J Biol Chem 269(44):27670-8	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2002-04-11 IDA: Inferred from Direct Assay Assigned on 2002-04-11	Lee SM and Park JW  (1998) Thermosensitive phenotype of yeast mutant lacking thioredoxin peroxidase. Arch Biochem Biophys 359(1):99-106	SGD
unfolded protein binding	IMP: Inferred from Mutant Phenotype Assigned on 2006-07-04	Rand JD and Grant CM  (2006) The thioredoxin system protects ribosomes against stress-induced aggregation. Mol Biol Cell 17(1):387-401	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell redox homeostasis	IMP: Inferred from Mutant Phenotype Assigned on 2002-04-11 IDA: Inferred from Direct Assay Assigned on 2002-04-11	Chae HZ, et al.  (1993) Cloning, sequencing, and mutation of thiol-specific antioxidant gene of Saccharomyces cerevisiae. J Biol Chem 268(22):16815-21	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2002-04-11	Lee SM and Park JW  (1998) Thermosensitive phenotype of yeast mutant lacking thioredoxin peroxidase. Arch Biochem Biophys 359(1):99-106	SGD
cellular response to oxidative stress	IMP: Inferred from Mutant Phenotype Assigned on 2002-04-11 IDA: Inferred from Direct Assay Assigned on 2002-04-11	Chae HZ, et al.  (1993) Cloning, sequencing, and mutation of thiol-specific antioxidant gene of Saccharomyces cerevisiae. J Biol Chem 268(22):16815-21	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2008-04-02	Trotter EW, et al.  (2008) The yeast Tsa1 peroxiredoxin is a ribosome-associated antioxidant. Biochem J 412(1):73-80	SGD
	IGI: Inferred from Genetic Interaction with SGD:PRX1, SGD:AHP1, SGD:DOT5, SGD:TSA2 Assigned on 2006-08-11	Wong CM, et al.  (2004) Peroxiredoxin-null yeast cells are hypersensitive to oxidative stress and are genomically unstable. J Biol Chem 279(22):23207-13	SGD
DNA damage checkpoint	IGI: Inferred from Genetic Interaction with SGD:DUN1, SGD:RAD53, SGD:RFX1, SGD:SML1 Assigned on 2009-11-04	Tang HM, et al.  (2009) Loss of Yeast Peroxiredoxin Tsa1p Induces Genome Instability through Activation of the DNA Damage Checkpoint and Elevation of dNTP Levels. PLoS Genet 5(10):e1000697	SGD
DNA protection	IMP: Inferred from Mutant Phenotype Assigned on 2009-06-29	Iraqui I, et al.  (2009) Peroxiredoxin Tsa1 is the key peroxidase suppressing genome instability and protecting against cell death in Saccharomyces cerevisiae. PLoS Genet 5(6):e1000524	SGD
protein folding	IMP: Inferred from Mutant Phenotype Assigned on 2010-01-27	Rand JD and Grant CM  (2006) The thioredoxin system protects ribosomes against stress-induced aggregation. Mol Biol Cell 17(1):387-401	SGD
replicative cell aging	IMP: Inferred from Mutant Phenotype Assigned on 2010-12-02	Timmermann B, et al.  (2010) A new dominant peroxiredoxin allele identified by whole-genome re-sequencing of random mutagenized yeast causes oxidant-resistance and premature aging. Aging (Albany NY) 2(8):475-486	SGD
response to hydroperoxide	IMP: Inferred from Mutant Phenotype Assigned on 2008-04-02	Munhoz DC and Netto LE  (2004) Cytosolic thioredoxin peroxidase I and II are important defenses of yeast against organic hydroperoxide insult: catalases and peroxiredoxins cooperate in the decomposition of H2O2 by yeast. J Biol Chem 279(34):35219-27	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2002-04-11	Chae HZ, et al.  (1993) Cloning, sequencing, and mutation of thiol-specific antioxidant gene of Saccharomyces cerevisiae. J Biol Chem 268(22):16815-21	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-06-24	Park SG, et al.  (2000) Distinct physiological functions of thiol peroxidase isoenzymes in Saccharomyces cerevisiae. J Biol Chem 275(8):5723-32	SGD
cytosol	IDA: Inferred from Direct Assay Assigned on 2008-04-02	Trotter EW, et al.  (2008) The yeast Tsa1 peroxiredoxin is a ribosome-associated antioxidant. Biochem J 412(1):73-80	SGD
polysome	IDA: Inferred from Direct Assay Assigned on 2008-04-02	Trotter EW, et al.  (2008) The yeast Tsa1 peroxiredoxin is a ribosome-associated antioxidant. Biochem J 412(1):73-80	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2012-12-12	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
antioxidant activity	IEA: Inferred from Electronic Annotation with EBI:IPR000866 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0049 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
oxidoreductase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000866 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0560 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
peroxidase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0575 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
peroxiredoxin activity	IEA: Inferred from Electronic Annotation with EBI:IPR019479 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:1.11.1.15 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
oxidation-reduction process	IEA: Inferred from Electronic Annotation with EBI:IPR019479, EBI:IPR000866 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0560 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).