This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2002-08-17    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
GTPase activity	IDA: Inferred from Direct Assay Assigned on 2010-03-24	Will E, et al.  (2001) Expression, purification, and biochemical properties of Ypt/Rab GTPase-activating proteins of Gyp family. Methods Enzymol 329:50-8	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
endocytosis	IMP: Inferred from Mutant Phenotype Assigned on 2006-08-17	Wichmann H, et al.  (1992) Endocytosis in yeast: evidence for the involvement of a small GTP-binding protein (Ypt7p). Cell 71(7):1131-42	SGD
Golgi to vacuole transport	TAS: Traceable Author Statement Assigned on 2001-01-18	Conibear E and Stevens TH  (1998) Multiple sorting pathways between the late Golgi and the vacuole in yeast. Biochim Biophys Acta 1404(1-2):211-30	SGD
piecemeal microautophagy of nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2008-08-28	Krick R, et al.  (2008) Piecemeal microautophagy of the nucleus requires the core macroautophagy genes. Mol Biol Cell 19(10):4492-505	SGD
protein localization to vacuole	IMP: Inferred from Mutant Phenotype Assigned on 2012-09-26	Liu TT, et al.  (2012) Rab GTPase regulation of retromer-mediated cargo export during endosome maturation. Mol Biol Cell 23(13):2505-15	SGD
regulation of vacuole fusion, non-autophagic	IMP: Inferred from Mutant Phenotype Assigned on 2009-11-13	Eitzen G, et al.  (2001) Rho1p and Cdc42p act after Ypt7p to regulate vacuole docking. EMBO J 20(20):5650-6	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-11-13	Haas A, et al.  (1995) The GTPase Ypt7p of Saccharomyces cerevisiae is required on both partner vacuoles for the homotypic fusion step of vacuole inheritance. EMBO J 14(21):5258-70	SGD
retrograde transport, endosome to Golgi	IPI: Inferred from Physical Interaction with SGD:VPS35 Assigned on 2012-09-26	Liu TT, et al.  (2012) Rab GTPase regulation of retromer-mediated cargo export during endosome maturation. Mol Biol Cell 23(13):2505-15	SGD
vacuole inheritance	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Haas A, et al.  (1995) The GTPase Ypt7p of Saccharomyces cerevisiae is required on both partner vacuoles for the homotypic fusion step of vacuole inheritance. EMBO J 14(21):5258-70	SGD
vesicle-mediated transport	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Mayer A and Wickner W  (1997) Docking of yeast vacuoles is catalyzed by the Ras-like GTPase Ypt7p after symmetric priming by Sec18p (NSF). J Cell Biol 136(2):307-17	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
fungal-type vacuole	IDA: Inferred from Direct Assay Assigned on 2007-04-30	Wang L, et al.  (2002) Vacuole fusion at a ring of vertex docking sites leaves membrane fragments within the organelle. Cell 108(3):357-69	SGD
fungal-type vacuole membrane	IDA: Inferred from Direct Assay Assigned on 2012-09-26	Liu TT, et al.  (2012) Rab GTPase regulation of retromer-mediated cargo export during endosome maturation. Mol Biol Cell 23(13):2505-15	SGD
colocalizes_with retromer complex, inner shell	IPI: Inferred from Physical Interaction with SGD:PEP8, SGD:VPS35 Assigned on 2012-09-26	Liu TT, et al.  (2012) Rab GTPase regulation of retromer-mediated cargo export during endosome maturation. Mol Biol Cell 23(13):2505-15	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrial outer membrane	IDA: Inferred from Direct Assay Assigned on 2006-03-17	Zahedi RP, et al.  (2006) Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins. Mol Biol Cell 17(3):1436-50	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
GTP binding	IEA: Inferred from Electronic Annotation with EBI:IPR001806, EBI:IPR005225, EBI:IPR003579 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0342 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
protein transport	IEA: Inferred from Electronic Annotation with EBI:IPR003579 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
small GTPase mediated signal transduction	IEA: Inferred from Electronic Annotation with EBI:IPR001806, EBI:IPR003579 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
vacuole	IEA: Inferred from Electronic Annotation with EBI:SL-0272 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0926 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).