This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2002-10-30    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
Rho guanyl-nucleotide exchange factor activity	IDA: Inferred from Direct Assay Assigned on 2002-10-30	Schmelzle T, et al.  (2002) Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeast. Mol Cell Biol 22(5):1329-39	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
fungal-type cell wall organization	IGI: Inferred from Genetic Interaction Assigned on 2002-10-30 IMP: Inferred from Mutant Phenotype Assigned on 2002-10-30	Schmelzle T, et al.  (2002) Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeast. Mol Cell Biol 22(5):1329-39	SGD
signal transduction	IGI: Inferred from Genetic Interaction Assigned on 2002-10-30 IMP: Inferred from Mutant Phenotype Assigned on 2002-10-30	Schmelzle T, et al.  (2002) Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeast. Mol Cell Biol 22(5):1329-39	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular bud neck	IDA: Inferred from Direct Assay Assigned on 2012-03-17	Yoshida S, et al.  (2006) Polo-like kinase Cdc5 controls the local activation of Rho1 to promote cytokinesis. Science 313(5783):108-11	SGD
incipient cellular bud site	IDA: Inferred from Direct Assay Assigned on 2012-03-17	Kono K, et al.  (2008) G1/S Cyclin-dependent Kinase Regulates Small GTPase Rho1p through Phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae. Mol Biol Cell 19(4):1763-1771	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for TUS1

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
guanyl-nucleotide exchange factor activity	IEA: Inferred from Electronic Annotation with EBI:KW-0344 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
phospholipid binding	IEA: Inferred from Electronic Annotation with EBI:IPR001849 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
Rho guanyl-nucleotide exchange factor activity	IEA: Inferred from Electronic Annotation with EBI:IPR000219 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
small GTPase regulator activity	IEA: Inferred from Electronic Annotation with EBI:IPR001180 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
regulation of Rho protein signal transduction	IEA: Inferred from Electronic Annotation with EBI:IPR000219 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
intracellular	IEA: Inferred from Electronic Annotation with EBI:IPR000219 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).