This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2007-06-22    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
1,3-beta-D-glucan synthase activity	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Inoue SB, et al.  (1995) Characterization and gene cloning of 1,3-beta-D-glucan synthase from Saccharomyces cerevisiae. Eur J Biochem 231(3):845-54	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2007-06-22 IDA: Inferred from Direct Assay Assigned on 2007-06-22	Douglas CM, et al.  (1994) The Saccharomyces cerevisiae FKS1 (ETG1) gene encodes an integral membrane protein which is a subunit of 1,3-beta-D-glucan synthase. Proc Natl Acad Sci U S A 91(26):12907-11	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
(1->3)-beta-D-glucan biosynthetic process	IMP: Inferred from Mutant Phenotype Assigned on 2007-06-22 IDA: Inferred from Direct Assay Assigned on 2007-06-22	Douglas CM, et al.  (1994) The Saccharomyces cerevisiae FKS1 (ETG1) gene encodes an integral membrane protein which is a subunit of 1,3-beta-D-glucan synthase. Proc Natl Acad Sci U S A 91(26):12907-11	SGD
positive regulation of endocytosis	IMP: Inferred from Mutant Phenotype Assigned on 2009-11-13	deHart AK, et al.  (2003) Receptor internalization in yeast requires the Tor2-Rho1 signaling pathway. Mol Biol Cell 14(11):4676-84	SGD
regulation of cell size	IMP: Inferred from Mutant Phenotype Assigned on 2007-02-21	Kikuchi Y, et al.  (2007) Involvement of Rho-type GTPase in control of cell size in Saccharomyces cerevisiae. FEMS Yeast Res 7(4):569-78	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
1,3-beta-D-glucan synthase complex	IDA: Inferred from Direct Assay Assigned on 2007-06-22	Mol PC, et al.  (1994) A GTP-binding protein regulates the activity of (1-->3)-beta-glucan synthase, an enzyme directly involved in yeast cell wall morphogenesis. J Biol Chem 269(49):31267-74	SGD
actin cortical patch	IDA: Inferred from Direct Assay Assigned on 2005-02-16	Utsugi T, et al.  (2002) Movement of yeast 1,3-beta-glucan synthase is essential for uniform cell wall synthesis. Genes Cells 7(1):1-9	SGD
cellular bud neck	IDA: Inferred from Direct Assay Assigned on 2012-08-13	Utsugi T, et al.  (2002) Movement of yeast 1,3-beta-glucan synthase is essential for uniform cell wall synthesis. Genes Cells 7(1):1-9	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-08-13	Qadota H, et al.  (1996) Identification of yeast Rho1p GTPase as a regulatory subunit of 1,3-beta-glucan synthase. Science 272(5259):279-81	SGD
cellular bud tip	IDA: Inferred from Direct Assay Assigned on 2012-08-13	Utsugi T, et al.  (2002) Movement of yeast 1,3-beta-glucan synthase is essential for uniform cell wall synthesis. Genes Cells 7(1):1-9	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-08-13	Qadota H, et al.  (1996) Identification of yeast Rho1p GTPase as a regulatory subunit of 1,3-beta-glucan synthase. Science 272(5259):279-81	SGD
plasma membrane	IDA: Inferred from Direct Assay Assigned on 2012-08-13	Utsugi T, et al.  (2002) Movement of yeast 1,3-beta-glucan synthase is essential for uniform cell wall synthesis. Genes Cells 7(1):1-9	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-08-13	Aronova S, et al.  (2007) Probing the Membrane Environment of the TOR Kinases Reveals Functional Interactions between TORC1, Actin, and Membrane Trafficking in Saccharomyces cerevisiae. Mol Biol Cell 18(8):2779-94	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	ISM: Inferred from Sequence Model Assigned on 2011-05-31	De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70	SGD
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
1,3-beta-D-glucan synthase activity	IEA: Inferred from Electronic Annotation with EBI:IPR003440 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:2.4.1.34 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
transferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity, transferring glycosyl groups	IEA: Inferred from Electronic Annotation with EBI:KW-0328 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
(1->3)-beta-D-glucan biosynthetic process	IEA: Inferred from Electronic Annotation with EBI:IPR003440 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
regulation of cell shape	IEA: Inferred from Electronic Annotation with EBI:KW-0133 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
1,3-beta-D-glucan synthase complex	IEA: Inferred from Electronic Annotation with EBI:IPR003440 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
integral to membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0812 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:IPR003440 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitochondrion	IEA: Inferred from Electronic Annotation with EBI:SL-0173 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0496 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
plasma membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0039 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-1003 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).