This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2012-12-10    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleocytoplasmic transporter activity	IPI: Inferred from Physical Interaction with SGD:KAP95 Assigned on 2012-06-18	Patel SS, et al.  (2007) Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex. Cell 129(1):83-96	SGD
	IGI: Inferred from Genetic Interaction Assigned on 2012-12-10 IPI: Inferred from Physical Interaction with SGD:SRP1 Assigned on 2012-12-10	Solsbacher J, et al.  (2000) Nup2p, a yeast nucleoporin, functions in bidirectional transport of importin alpha. Mol Cell Biol 20(22):8468-79	SGD
	IPI: Inferred from Physical Interaction with SGD:KAP122 Assigned on 2012-06-18	Titov AA and Blobel G  (1999) The karyopherin Kap122p/Pdr6p imports both subunits of the transcription factor IIA into the nucleus. J Cell Biol 147(2):235-46	SGD
	IPI: Inferred from Physical Interaction with SGD:KAP95 Assigned on 2012-06-18	Pyhtila B and Rexach M  (2003) A gradient of affinity for the karyopherin Kap95p along the yeast nuclear pore complex. J Biol Chem 278(43):42699-709	SGD
	IGI: Inferred from Genetic Interaction with SGD:GSP1, SGD:KAP95 Assigned on 2012-06-19	Allen NP, et al.  (2001) Proteomic analysis of nucleoporin interacting proteins. J Biol Chem 276(31):29268-74	SGD
Ran GTPase binding	IPI: Inferred from Physical Interaction with SGD:GSP1 Assigned on 2012-12-10	Dingwall C, et al.  (1995) A family of Ran binding proteins that includes nucleoporins. Proc Natl Acad Sci U S A 92(16):7525-9	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin silencing at silent mating-type cassette	IMP: Inferred from Mutant Phenotype Assigned on 2012-06-18	Ruben GJ, et al.  (2011) Nucleoporin Mediated Nuclear Positioning and Silencing of HMR. PLoS One 6(7):e21923	SGD
maintenance of chromatin silencing at telomere	IMP: Inferred from Mutant Phenotype Assigned on 2012-12-10	Dilworth DJ, et al.  (2005) The mobile nucleoporin Nup2p and chromatin-bound Prp20p function in endogenous NPC-mediated transcriptional control. J Cell Biol 171(6):955-65	SGD
mRNA export from nucleus in response to heat stress	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-23	Thomsen R, et al.  (2008) General, rapid, and transcription-dependent fragmentation of nucleolar antigens in S. cerevisiae mRNA export mutants. RNA 14(4):706-16	SGD
NLS-bearing substrate import into nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2012-12-10	Solsbacher J, et al.  (2000) Nup2p, a yeast nucleoporin, functions in bidirectional transport of importin alpha. Mol Cell Biol 20(22):8468-79	SGD
	IGI: Inferred from Genetic Interaction with SGD:NUP60, SGD:NUP1, SGD:NUP159, SGD:NUP42 Assigned on 2011-05-25	Strawn LA, et al.  (2004) Minimal nuclear pore complexes define FG repeat domains essential for transport. Nat Cell Biol 6(3):197-206	SGD
poly(A)+ mRNA export from nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-23	Powrie EA, et al.  (2011) A nucleoporin, Nup60p, affects the nuclear and cytoplasmic localization of ASH1 mRNA in S. cerevisiae. RNA 17(1):134-44	SGD
posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-23	Light WH, et al.  (2010) Interaction of a DNA Zip code with the nuclear pore complex promotes H2A.Z incorporation and INO1 transcriptional memory. Mol Cell 40(1):112-25	SGD
protein export from nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2012-12-10	Solsbacher J, et al.  (2000) Nup2p, a yeast nucleoporin, functions in bidirectional transport of importin alpha. Mol Cell Biol 20(22):8468-79	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2012-12-10 IPI: Inferred from Physical Interaction with SGD:SRP1 Assigned on 2012-12-10	Hood JK, et al.  (2000) Nup2p is located on the nuclear side of the nuclear pore complex and coordinates Srp1p/importin-alpha export. J Cell Sci 113 ( Pt 8):1471-80	SGD
	IGI: Inferred from Genetic Interaction with SGD:SRM1, SGD:SRP1 Assigned on 2012-12-10 IMP: Inferred from Mutant Phenotype Assigned on 2012-12-10	Booth JW, et al.  (1999) The yeast nucleoporin Nup2p is involved in nuclear export of importin alpha/Srp1p. J Biol Chem 274(45):32360-7	SGD
protein import into nucleus, substrate release	IDA: Inferred from Direct Assay Assigned on 2012-12-10	Solsbacher J, et al.  (2000) Nup2p, a yeast nucleoporin, functions in bidirectional transport of importin alpha. Mol Cell Biol 20(22):8468-79	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-12-10	Gilchrist D, et al.  (2002) Accelerating the rate of disassembly of karyopherin.cargo complexes. J Biol Chem 277(20):18161-72	SGD
protein targeting to nuclear inner membrane	IMP: Inferred from Mutant Phenotype Assigned on 2012-12-10	King MC, et al.  (2006) Karyopherin-mediated import of integral inner nuclear membrane proteins. Nature 442(7106):1003-7	SGD
transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-23	Light WH, et al.  (2010) Interaction of a DNA Zip code with the nuclear pore complex promotes H2A.Z incorporation and INO1 transcriptional memory. Mol Cell 40(1):112-25	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nuclear pore cytoplasmic filaments	IDA: Inferred from Direct Assay Assigned on 2012-12-10	Dilworth DJ, et al.  (2001) Nup2p dynamically associates with the distal regions of the yeast nuclear pore complex. J Cell Biol 153(7):1465-78	SGD
nuclear pore nuclear basket	IDA: Inferred from Direct Assay Assigned on 2012-12-10	Dilworth DJ, et al.  (2001) Nup2p dynamically associates with the distal regions of the yeast nuclear pore complex. J Cell Biol 153(7):1465-78	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-12-10	Hood JK, et al.  (2000) Nup2p is located on the nuclear side of the nuclear pore complex and coordinates Srp1p/importin-alpha export. J Cell Sci 113 ( Pt 8):1471-80	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	ISM: Inferred from Sequence Model Assigned on 2011-05-31	De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70	SGD
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-09-28	Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
intracellular transport	IEA: Inferred from Electronic Annotation with EBI:IPR000156 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
mRNA transport	IEA: Inferred from Electronic Annotation with EBI:KW-0509 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein transport	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813, EBI:KW-0811 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nuclear membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0182 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
nuclear pore	IEA: Inferred from Electronic Annotation with EBI:IPR015007 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0185 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0906 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).