This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-06-19    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
contributes_to telomerase activity	IDA: Inferred from Direct Assay Assigned on 2005-04-21	Liao XH, et al.  (2005) Characterization of recombinant Saccharomyces cerevisiae telomerase core enzyme purified from yeast. Biochem J 390(Pt 1):169-76	SGD
telomeric DNA binding	IDA: Inferred from Direct Assay Assigned on 2007-08-06	Sabourin M, et al.  (2007) Telomerase and Tel1p preferentially associate with short telomeres in S. cerevisiae. Mol Cell 27(4):550-61	SGD
telomeric template RNA reverse transcriptase activity	TAS: Traceable Author Statement Assigned on 2002-06-25	Livengood AJ, et al.  (2002) Essential regions of Saccharomyces cerevisiae telomerase RNA: separate elements for Est1p and Est2p interaction. Mol Cell Biol 22(7):2366-74	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
replicative cell aging	IGI: Inferred from Genetic Interaction with SGD:DNL4 Assigned on 2008-07-30	Meyer DH and Bailis AM  (2008) Mating type influences chromosome loss and replicative senescence in telomerase-deficient budding yeast by Dnl4-dependent telomere fusion. Mol Microbiol 69(5):1246-54	SGD
telomere maintenance via telomerase	IMP: Inferred from Mutant Phenotype Assigned on 2005-04-21 IDA: Inferred from Direct Assay Assigned on 2005-04-21	Liao XH, et al.  (2005) Characterization of recombinant Saccharomyces cerevisiae telomerase core enzyme purified from yeast. Biochem J 390(Pt 1):169-76	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2008-09-18	Lendvay TS, et al.  (1996) Senescence mutants of Saccharomyces cerevisiae with a defect in telomere replication identify three additional EST genes. Genetics 144(4):1399-412	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleolus	IDA: Inferred from Direct Assay Assigned on 2002-08-28	Teixeira MT, et al.  (2002) Intracellular trafficking of yeast telomerase components. EMBO Rep 3(7):652-9	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2002-08-28	Teixeira MT, et al.  (2002) Intracellular trafficking of yeast telomerase components. EMBO Rep 3(7):652-9	SGD
telomerase catalytic core complex	IDA: Inferred from Direct Assay Assigned on 2005-04-21 IPI: Inferred from Physical Interaction Assigned on 2005-04-21	Liao XH, et al.  (2005) Characterization of recombinant Saccharomyces cerevisiae telomerase core enzyme purified from yeast. Biochem J 390(Pt 1):169-76	SGD
telomerase holoenzyme complex	IDA: Inferred from Direct Assay Assigned on 2003-06-19 IPI: Inferred from Physical Interaction Assigned on 2003-06-19	Hughes TR, et al.  (2000) The Est3 protein is a subunit of yeast telomerase. Curr Biol 10(13):809-12	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for EST2

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR003545 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotidyltransferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0548 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
RNA binding	IEA: Inferred from Electronic Annotation with EBI:IPR000477 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
RNA-directed DNA polymerase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000477, EBI:IPR021891 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0695 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with IUBMB:2.7.7.49 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
telomeric template RNA reverse transcriptase activity	IEA: Inferred from Electronic Annotation with EBI:IPR003545 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
transferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
RNA-dependent DNA replication	IEA: Inferred from Electronic Annotation with EBI:IPR000477 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
chromosome	IEA: Inferred from Electronic Annotation with EBI:KW-0158 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
chromosome, telomeric region	IEA: Inferred from Electronic Annotation with EBI:SL-0276 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0779 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:IPR003545 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).