This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2002-11-04    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA binding	IDA: Inferred from Direct Assay Assigned on 2002-11-03	Foiani M, et al.  (2000) DNA damage checkpoints and DNA replication controls in Saccharomyces cerevisiae. Mutat Res 451(1-2):187-96	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin silencing at telomere	IMP: Inferred from Mutant Phenotype Assigned on 2002-08-21	Corda Y, et al.  (1999) Interaction between Set1p and checkpoint protein Mec3p in DNA repair and telomere functions. Nat Genet 21(2):204-8	SGD
DNA damage checkpoint	IMP: Inferred from Mutant Phenotype Assigned on 2002-08-21	Paulovich AG, et al.  (1998) The Saccharomyces cerevisiae RAD9, RAD17, RAD24 and MEC3 genes are required for tolerating irreparable, ultraviolet-induced DNA damage. Genetics 150(1):75-93	SGD
DNA metabolic process	TAS: Traceable Author Statement Assigned on 2002-11-03	Longhese MP, et al.  (2000) Checkpoint proteins influence telomeric silencing and length maintenance in budding yeast. Genetics 155(4):1577-91	SGD
telomere maintenance via recombination	IGI: Inferred from Genetic Interaction with SGD:TLC1 Assigned on 2007-02-22	Grandin N and Charbonneau M  (2007) Control of the yeast telomeric senescence survival pathways of recombination by the Mec1 and Mec3 DNA damage sensors and RPA. Nucleic Acids Res 35(3):822-38	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
checkpoint clamp complex	IDA: Inferred from Direct Assay Assigned on 2008-08-13	Kondo T, et al.  (1999) Role of a complex containing Rad17, Mec3, and Ddc1 in the yeast DNA damage checkpoint pathway. Mol Cell Biol 19(2):1136-43	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-08-13	Majka J and Burgers PM  (2003) Yeast Rad17/Mec3/Ddc1: a sliding clamp for the DNA damage checkpoint. Proc Natl Acad Sci U S A 100(5):2249-54	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-08-13	Paciotti V, et al.  (1998) Mec1p is essential for phosphorylation of the yeast DNA damage checkpoint protein Ddc1p, which physically interacts with Mec3p. EMBO J 17(14):4199-209	SGD
nucleus	TAS: Traceable Author Statement Assigned on 2002-11-04	Clarke DJ and Gimenez-Abian JF  (2000) Checkpoints controlling mitosis. Bioessays 22(4):351-63	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for MEC3

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA binding	IEA: Inferred from Electronic Annotation with EBI:KW-0238 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DNA damage checkpoint	IEA: Inferred from Electronic Annotation with EBI:IPR007150 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
DNA repair	IEA: Inferred from Electronic Annotation with EBI:IPR007150 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0234 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
response to DNA damage stimulus	IEA: Inferred from Electronic Annotation with EBI:KW-0227 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
checkpoint clamp complex	IEA: Inferred from Electronic Annotation with EBI:IPR007150 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).