This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-06-09    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
1-phosphatidylinositol-3-kinase activity	IMP: Inferred from Mutant Phenotype Assigned on 2008-06-09 IDA: Inferred from Direct Assay Assigned on 2008-06-09	Schu PV, et al.  (1993) Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting. Science 260(5104):88-91	SGD
	IDA: Inferred from Direct Assay Assigned on 2001-02-02	Stack JH and Emr SD  (1994) Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol-specific PI 3-kinase activities. J Biol Chem 269(50):31552-62	SGD
protein kinase activity	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Stack JH and Emr SD  (1994) Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol-specific PI 3-kinase activities. J Biol Chem 269(50):31552-62	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
peroxisome degradation	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-11	Grunau S, et al.  (2011) The phosphoinositide 3-kinase Vps34p is required for pexophagy in Saccharomyces cerevisiae. Biochem J 434(1):161-170	SGD
phosphatidylinositol phosphorylation	IMP: Inferred from Mutant Phenotype Assigned on 2008-06-09 IDA: Inferred from Direct Assay Assigned on 2008-06-09	Schu PV, et al.  (1993) Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting. Science 260(5104):88-91	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-01-11	Stack JH and Emr SD  (1994) Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol-specific PI 3-kinase activities. J Biol Chem 269(50):31552-62	SGD
positive regulation of transcription elongation from RNA polymerase II promoter	IMP: Inferred from Mutant Phenotype Assigned on 2013-03-15	Gaur NA, et al.  (2013) Vps factors are required for efficient transcription elongation in budding yeast. Genetics 193(3):829-51	SGD
protein phosphorylation	IDA: Inferred from Direct Assay Assigned on 2001-01-18	Stack JH and Emr SD  (1994) Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol-specific PI 3-kinase activities. J Biol Chem 269(50):31552-62	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
endosome	IDA: Inferred from Direct Assay Assigned on 2008-06-09	Obara K, et al.  (2006) Assortment of phosphatidylinositol 3-kinase complexes--Atg14p directs association of complex I to the pre-autophagosomal structure in Saccharomyces cerevisiae. Mol Biol Cell 17(4):1527-39	SGD
fungal-type vacuole membrane	IDA: Inferred from Direct Assay Assigned on 2008-06-09	Obara K, et al.  (2006) Assortment of phosphatidylinositol 3-kinase complexes--Atg14p directs association of complex I to the pre-autophagosomal structure in Saccharomyces cerevisiae. Mol Biol Cell 17(4):1527-39	SGD
colocalizes_with nuclear pore	IDA: Inferred from Direct Assay Assigned on 2013-03-15	Gaur NA, et al.  (2013) Vps factors are required for efficient transcription elongation in budding yeast. Genetics 193(3):829-51	SGD
nucleus-vacuole junction	IDA: Inferred from Direct Assay Assigned on 2013-03-15	Gaur NA, et al.  (2013) Vps factors are required for efficient transcription elongation in budding yeast. Genetics 193(3):829-51	SGD
peroxisome	IDA: Inferred from Direct Assay Assigned on 2011-02-11	Grunau S, et al.  (2011) The phosphoinositide 3-kinase Vps34p is required for pexophagy in Saccharomyces cerevisiae. Biochem J 434(1):161-170	SGD
phosphatidylinositol 3-kinase complex I	IDA: Inferred from Direct Assay Assigned on 2008-05-20	Kihara A, et al.  (2001) Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae. J Cell Biol 152(3):519-30	SGD
phosphatidylinositol 3-kinase complex II	IDA: Inferred from Direct Assay Assigned on 2008-05-20	Kihara A, et al.  (2001) Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae. J Cell Biol 152(3):519-30	SGD
pre-autophagosomal structure	IDA: Inferred from Direct Assay Assigned on 2008-06-09	Obara K, et al.  (2006) Assortment of phosphatidylinositol 3-kinase complexes--Atg14p directs association of complex I to the pre-autophagosomal structure in Saccharomyces cerevisiae. Mol Biol Cell 17(4):1527-39	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for VPS34

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
1-phosphatidylinositol-3-kinase activity	IEA: Inferred from Electronic Annotation with EBI:IPR008290 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:2.7.1.137 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
ATP binding	IEA: Inferred from Electronic Annotation with EBI:KW-0067 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
kinase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0418 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
phosphotransferase activity, alcohol group as acceptor	IEA: Inferred from Electronic Annotation with EBI:IPR000403, EBI:IPR018936 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
transferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity, transferring phosphorus-containing groups	IEA: Inferred from Electronic Annotation with EBI:IPR011009 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
phosphatidylinositol phosphorylation	IEA: Inferred from Electronic Annotation with EBI:IPR015433, EBI:IPR008290 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
phosphatidylinositol-3-phosphate biosynthetic process	IEA: Inferred from Electronic Annotation with EBI:IPR008290 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	GOC
	IEA: Inferred from Electronic Annotation with IUBMB:2.7.1.137 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	GOC
phosphatidylinositol-mediated signaling	IEA: Inferred from Electronic Annotation with EBI:IPR015433, EBI:IPR008290 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
phosphorylation	IEA: Inferred from Electronic Annotation with EBI:KW-0418 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein transport	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
endosome	IEA: Inferred from Electronic Annotation with EBI:KW-0967 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
endosome membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0100 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
Golgi apparatus	IEA: Inferred from Electronic Annotation with EBI:SL-0132 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0333 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).