This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2012-06-11    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
structural molecule activity	IMP: Inferred from Mutant Phenotype Assigned on 2012-06-11	Copic A, et al.  (2012) ER cargo properties specify a requirement for COPII coat rigidity mediated by Sec13p. Science 335(6074):1359-62	SGD
	IDA: Inferred from Direct Assay Assigned on 2009-12-15	Fath S, et al.  (2007) Structure and organization of coat proteins in the COPII cage. Cell 129(7):1325-36	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-06-11	Antonny B, et al.  (2003) Self-assembly of minimal COPII cages. EMBO Rep 4(4):419-24	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
COPII-coated vesicle budding	IMP: Inferred from Mutant Phenotype Assigned on 2012-06-11	Pryer NK, et al.  (1993) Cytosolic Sec13p complex is required for vesicle formation from the endoplasmic reticulum in vitro. J Cell Biol 120(4):865-75	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2012-06-11	Salama NR, et al.  (1993) The Sec13p complex and reconstitution of vesicle budding from the ER with purified cytosolic proteins. EMBO J 12(11):4073-82	SGD
ER-associated protein catabolic process	IMP: Inferred from Mutant Phenotype Assigned on 2012-06-11	Fu L and Sztul E  (2003) Traffic-independent function of the Sar1p/COPII machinery in proteasomal sorting of the cystic fibrosis transmembrane conductance regulator. J Cell Biol 160(2):157-63	SGD
nuclear pore distribution	IMP: Inferred from Mutant Phenotype Assigned on 2012-06-11	Siniossoglou S, et al.  (2000) Structure and assembly of the Nup84p complex. J Cell Biol 149(1):41-54	SGD
positive regulation of GTPase activity	IDA: Inferred from Direct Assay Assigned on 2009-12-15	Antonny B, et al.  (2001) Dynamics of the COPII coat with GTP and stable analogues. Nat Cell Biol 3(6):531-7	SGD
positive regulation of transcription, DNA-dependent	IDA: Inferred from Direct Assay Assigned on 2012-06-07	Menon BB, et al.  (2005) Reverse recruitment: the Nup84 nuclear pore subcomplex mediates Rap1/Gcr1/Gcr2 transcriptional activation. Proc Natl Acad Sci U S A 102(16):5749-54	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
COPII vesicle coat	IDA: Inferred from Direct Assay Assigned on 2012-06-11	Matsuoka K, et al.  (2001) Surface structure of the COPII-coated vesicle. Proc Natl Acad Sci U S A 98(24):13705-9	SGD
	IDA: Inferred from Direct Assay Assigned on 2009-12-15	Barlowe C, et al.  (1994) COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum. Cell 77(6):895-907	SGD
nuclear pore outer ring	IDA: Inferred from Direct Assay Assigned on 2012-06-07	Alber F, et al.  (2007) The molecular architecture of the nuclear pore complex. Nature 450(7170):695-701	SGD
	IDA: Inferred from Direct Assay Assigned on 2009-10-09	Lutzmann M, et al.  (2002) Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins. EMBO J 21(3):387-97	SGD
Seh1-associated complex	IDA: Inferred from Direct Assay Assigned on 2011-05-19	Dokudovskaya S, et al.  (2011) A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae. Mol Cell Proteomics 10(6):M110.006478	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for SEC13

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
mRNA transport	IEA: Inferred from Electronic Annotation with EBI:KW-0509 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein transport	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:KW-0813, EBI:KW-0811 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
vesicle-mediated transport	IEA: Inferred from Electronic Annotation with EBI:KW-0931 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasmic vesicle	IEA: Inferred from Electronic Annotation with EBI:KW-0968 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
endoplasmic reticulum	IEA: Inferred from Electronic Annotation with EBI:KW-0256 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
endoplasmic reticulum membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0097 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
ER to Golgi transport vesicle membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0078 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nuclear pore	IEA: Inferred from Electronic Annotation with EBI:SL-0185 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0906 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleus	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
vacuolar membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0271 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
vacuole	IEA: Inferred from Electronic Annotation with EBI:KW-0926 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).