This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-05-08    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
chromatin binding	IDA: Inferred from Direct Assay Assigned on 2007-08-15	Ricke RM and Bielinsky AK  (2004) Mcm10 regulates the stability and chromatin association of DNA polymerase-alpha. Mol Cell 16(2):173-85	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-09	Zou L and Stillman B  (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6	SGD
DNA replication origin binding	IDA: Inferred from Direct Assay Assigned on 2007-08-14	Sclafani RA, et al.  (2002) The mcm5-bob1 bypass of Cdc7p/Dbf4p in DNA replication depends on both Cdk1-independent and Cdk1-dependent steps in Saccharomyces cerevisiae. Genetics 161(1):47-57	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA replication initiation	IGI: Inferred from Genetic Interaction with SGD:MCM7 Assigned on 2007-08-02 IMP: Inferred from Mutant Phenotype Assigned on 2007-08-02	Hennessy KM, et al.  (1991) A group of interacting yeast DNA replication genes. Genes Dev 5(6):958-69	SGD
double-strand break repair via break-induced replication	IMP: Inferred from Mutant Phenotype Assigned on 2010-07-09	Lydeard JR, et al.  (2010) Break-induced replication requires all essential DNA replication factors except those specific for pre-RC assembly. Genes Dev 24(11):1133-44	SGD
pre-replicative complex assembly	IPI: Inferred from Physical Interaction Assigned on 2002-11-26	Aparicio OM, et al.  (1997) Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase. Cell 91(1):59-69	SGD
regulation of chromatin silencing at telomere	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-16	Rehman MA, et al.  (2006) Differential requirement of DNA replication factors for subtelomeric ARS consensus sequence protosilencers in Saccharomyces cerevisiae. Genetics 174(4):1801-10	SGD
S phase of mitotic cell cycle	IGI: Inferred from Genetic Interaction with SGD:MCM5, SGD:MCM7 Assigned on 2007-08-10 IMP: Inferred from Mutant Phenotype Assigned on 2007-08-10	Hennessy KM, et al.  (1991) A group of interacting yeast DNA replication genes. Genes Dev 5(6):958-69	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
DNA replication preinitiation complex	IDA: Inferred from Direct Assay Assigned on 2007-08-02	Zou L and Stillman B  (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6	SGD
nuclear replication fork	IDA: Inferred from Direct Assay Assigned on 2009-07-10	Calzada A, et al.  (2005) Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork. Genes Dev 19(16):1905-19	SGD
nucleus	IDA: Inferred from Direct Assay Assigned on 2007-08-02	Hopwood B and Dalton S  (1996) Cdc45p assembles into a complex with Cdc46p/Mcm5p, is required for minichromosome maintenance, and is essential for chromosomal DNA replication. Proc Natl Acad Sci U S A 93(22):12309-14	SGD
pre-replicative complex	IDA: Inferred from Direct Assay Assigned on 2002-11-26	Aparicio OM, et al.  (1997) Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase. Cell 91(1):59-69	SGD
replication fork protection complex	IDA: Inferred from Direct Assay Assigned on 2009-08-24	Gambus A, et al.  (2006) GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks. Nat Cell Biol 8(4):358-66	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for CDC45

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Biological Process | Cellular Component

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cell cycle	IEA: Inferred from Electronic Annotation with EBI:KW-0131 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DNA replication	IEA: Inferred from Electronic Annotation with EBI:KW-0235 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DNA replication initiation	IEA: Inferred from Electronic Annotation with EBI:IPR003874 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
nucleus	IEA: Inferred from Electronic Annotation with EBI:SL-0191 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0539 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).