This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2007-09-07    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ferric-chelate reductase activity	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-09-19	Yun CW, et al.  (2001) The role of the FRE family of plasma membrane reductases in the uptake of siderophore-iron in Saccharomyces cerevisiae. J Biol Chem 276(13):10218-23	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2007-09-07	Singh A, et al.  (2007) The metalloreductase Fre6p in Fe-efflux from the yeast vacuole. J Biol Chem 282(39):28619-26	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
copper ion import	IGI: Inferred from Genetic Interaction with SGD:CTR2 Assigned on 2007-08-06	Rees EM and Thiele DJ  (2007) Identification of a vacuole-associated metalloreductase and its role in Ctr2-mediated intracellular copper mobilization. J Biol Chem 282(30):21629-38	SGD
intracellular sequestering of iron ion	IMP: Inferred from Mutant Phenotype Assigned on 2007-09-07	Singh A, et al.  (2007) The metalloreductase Fre6p in Fe-efflux from the yeast vacuole. J Biol Chem 282(39):28619-26	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
fungal-type vacuole membrane	IDA: Inferred from Direct Assay Assigned on 2011-03-31	Rees EM and Thiele DJ  (2007) Identification of a vacuole-associated metalloreductase and its role in Ctr2-mediated intracellular copper mobilization. J Biol Chem 282(30):21629-38	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-03-31	Singh A, et al.  (2007) The metalloreductase Fre6p in Fe-efflux from the yeast vacuole. J Biol Chem 282(39):28619-26	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
fungal-type vacuole	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
integral to membrane	ISM: Inferred from Sequence Model Assigned on 2011-05-31	De Hertogh B, et al.  (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct Integr Genomics 2(4-5):154-70	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
metal ion binding	IEA: Inferred from Electronic Annotation with EBI:KW-0479 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
oxidoreductase activity	IEA: Inferred from Electronic Annotation with EBI:IPR017927, EBI:IPR013121, EBI:IPR013112 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0560 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
electron transport chain	IEA: Inferred from Electronic Annotation with EBI:KW-0249 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
ion transport	IEA: Inferred from Electronic Annotation with EBI:KW-0406 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
iron ion homeostasis	IEA: Inferred from Electronic Annotation with EBI:KW-0410 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
oxidation-reduction process	IEA: Inferred from Electronic Annotation with EBI:IPR017927, EBI:IPR013121, EBI:IPR013112 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0560 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
integral to membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0812 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
vacuolar membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0271 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
vacuole	IEA: Inferred from Electronic Annotation with EBI:KW-0926 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).