This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2008-05-14    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
Atg12 ligase activity	IMP: Inferred from Mutant Phenotype Assigned on 2005-10-17 IPI: Inferred from Physical Interaction with SGD:ATG12, SGD:ATG7 Assigned on 2005-10-17	Shintani T, et al.  (1999) Apg10p, a novel protein-conjugating enzyme essential for autophagy in yeast. EMBO J 18(19):5234-41	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular protein modification process	IMP: Inferred from Mutant Phenotype Assigned on 2005-10-17	Mizushima N, et al.  (1998) A protein conjugation system essential for autophagy. Nature 395(6700):395-8	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2005-10-17 IPI: Inferred from Physical Interaction with SGD:ATG12, SGD:ATG7 Assigned on 2005-10-17	Shintani T, et al.  (1999) Apg10p, a novel protein-conjugating enzyme essential for autophagy in yeast. EMBO J 18(19):5234-41	SGD
CVT pathway	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-14	Shintani T, et al.  (1999) Apg10p, a novel protein-conjugating enzyme essential for autophagy in yeast. EMBO J 18(19):5234-41	SGD
macroautophagy	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-14	Shintani T, et al.  (1999) Apg10p, a novel protein-conjugating enzyme essential for autophagy in yeast. EMBO J 18(19):5234-41	SGD
mitochondrion degradation	IMP: Inferred from Mutant Phenotype Assigned on 2010-01-07	Kanki T, et al.  (2009) A genomic screen for yeast mutants defective in selective mitochondria autophagy. Mol Biol Cell 20(22):4730-8	SGD
piecemeal microautophagy of nucleus	IMP: Inferred from Mutant Phenotype Assigned on 2008-08-28	Krick R, et al.  (2008) Piecemeal microautophagy of the nucleus requires the core macroautophagy genes. Mol Biol Cell 19(10):4492-505	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cellular_component unknown	ND: No Biological Data Available Assigned on 2008-05-14	SGD  (2002) Use of the ND evidence code for Gene Ontology (GO) terms in SGD ()	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for ATG10

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
ligase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0436 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
autophagy	IEA: Inferred from Electronic Annotation with EBI:IPR016524 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0072 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
protein transport	IEA: Inferred from Electronic Annotation with EBI:KW-0653 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transport	IEA: Inferred from Electronic Annotation with EBI:IPR016524 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0813 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:IPR016524 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).