This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2006-07-26    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
GTPase activity	IDA: Inferred from Direct Assay Assigned on 2006-07-26	Ingerman E, et al.  (2005) Dnm1 forms spirals that are structurally tailored to fit mitochondria. J Cell Biol 170(7):1021-7	SGD
identical protein binding	IDA: Inferred from Direct Assay Assigned on 2006-07-26	Ingerman E, et al.  (2005) Dnm1 forms spirals that are structurally tailored to fit mitochondria. J Cell Biol 170(7):1021-7	SGD
protein homodimerization activity	IDA: Inferred from Direct Assay Assigned on 2006-07-26	Ingerman E, et al.  (2005) Dnm1 forms spirals that are structurally tailored to fit mitochondria. J Cell Biol 170(7):1021-7	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
chronological cell aging	IMP: Inferred from Mutant Phenotype Assigned on 2008-09-23	Palermo V, et al.  (2007) Apoptosis and aging in mitochondrial morphology mutants of S. cerevisiae. Folia Microbiol (Praha) 52(5):479-83	SGD
mitochondrial fission	IGI: Inferred from Genetic Interaction with SGD:FZO1 Assigned on 2003-02-05 IMP: Inferred from Mutant Phenotype Assigned on 2003-02-05	Bleazard W, et al.  (1999) The dynamin-related GTPase Dnm1 regulates mitochondrial fission in yeast. Nat Cell Biol 1(5):298-304	SGD
mitochondrion inheritance	IGI: Inferred from Genetic Interaction with SGD:NUM1 Assigned on 2007-03-19	Cerveny KL, et al.  (2007) Yeast mitochondrial division and distribution require the cortical num1 protein. Dev Cell 12(3):363-75	SGD
mitochondrion localization	IGI: Inferred from Genetic Interaction with SGD:FZO1, SGD:NUM1 Assigned on 2013-05-15	Lackner LL, et al.  (2013) Endoplasmic reticulum-associated mitochondria-cortex tether functions in the distribution and inheritance of mitochondria. Proc Natl Acad Sci U S A 110(6):E458-67	SGD
mitochondrion organization	IMP: Inferred from Mutant Phenotype Assigned on 2005-04-26	Otsuga D, et al.  (1998) The dynamin-related GTPase, Dnm1p, controls mitochondrial morphology in yeast. J Cell Biol 143(2):333-49	SGD
peroxisome fission	IGI: Inferred from Genetic Interaction with SGD:VPS1 Assigned on 2010-01-26	Motley AM, et al.  (2008) Dnm1p-dependent peroxisome fission requires Caf4p, Mdv1p and Fis1p. J Cell Sci 121(Pt 10):1633-40	SGD
peroxisome organization	IGI: Inferred from Genetic Interaction with SGD:VPS1 Assigned on 2009-02-24 IMP: Inferred from Mutant Phenotype Assigned on 2009-02-24	Kuravi K, et al.  (2006) Dynamin-related proteins Vps1p and Dnm1p control peroxisome abundance in Saccharomyces cerevisiae. J Cell Sci 119(Pt 19):3994-4001	SGD
protein homooligomerization	IDA: Inferred from Direct Assay Assigned on 2006-07-26	Ingerman E, et al.  (2005) Dnm1 forms spirals that are structurally tailored to fit mitochondria. J Cell Biol 170(7):1021-7	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrial outer membrane	IDA: Inferred from Direct Assay Assigned on 2003-01-20	Tieu Q and Nunnari J  (2000) Mdv1p is a WD repeat protein that interacts with the dynamin-related GTPase, Dnm1p, to trigger mitochondrial division. J Cell Biol 151(2):353-66	SGD
peroxisome	IDA: Inferred from Direct Assay Assigned on 2009-02-24	Kuravi K, et al.  (2006) Dynamin-related proteins Vps1p and Dnm1p control peroxisome abundance in Saccharomyces cerevisiae. J Cell Sci 119(Pt 19):3994-4001	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
mitochondrion	IDA: Inferred from Direct Assay Assigned on 2006-12-12	Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Biological Process | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
GTP binding	IEA: Inferred from Electronic Annotation with EBI:IPR003130, EBI:IPR001401, EBI:IPR000375 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0342 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
GTPase activity	IEA: Inferred from Electronic Annotation with EBI:IPR003130, EBI:IPR001401 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
hydrolase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0378 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
nucleotide binding	IEA: Inferred from Electronic Annotation with EBI:KW-0547 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
GTP catabolic process	IEA: Inferred from Electronic Annotation with EBI:IPR003130, EBI:IPR001401 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	GOC

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
membrane	IEA: Inferred from Electronic Annotation with EBI:KW-0472 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitochondrial outer membrane	IEA: Inferred from Electronic Annotation with EBI:SL-0172 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-1000 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
mitochondrion	IEA: Inferred from Electronic Annotation with EBI:KW-0496 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).